School of Chemical Engineering, Shandong University of Technology, Zibo, 255049, PR China.
School of Chemical Engineering, Shandong University of Technology, Zibo, 255049, PR China.
Talanta. 2022 Jan 1;236:122865. doi: 10.1016/j.talanta.2021.122865. Epub 2021 Sep 10.
A sandwich-type electrochemical immunosensor was designed utilizing ferrocene-functionalized cuprous oxide superparticles (Au/Fc@CuO SPs) as the signal label and graphene supported by hollow carbon balls (HCNs-GR) as the substrate. The CuO SPs possess a superparticle structure with synergistic properties of isotropy and promising catalytic activity. Ferrocene (Fc) was deposited on the CuO SPs to act as the electronic transmission medium. The Au/Fc@CuO SPs played a pivotal role in improving the sensitivity of the immunosensor. The graphene supported by hollow carbon balls (HCNs-GR) was used to modify the electrode surface. The embedding of hollow carbon nanospheres (HCNs) reduced the decrease of the effective surface area caused by the stacking of graphene nanotubes. Meanwhile, the load of carbon balls further increases the surface area of graphene, enabled HCNs-GR to immobilize antibodies more effectively, improved the sensitivity of the immunosensor. The proposed immunosensor showed a linear range from 500 fg/mL to 100 ng/mL, with the detection limit to 25.7 fg/mL.
一种三明治型电化学免疫传感器，利用二茂铁功能化氧化亚铜超粒子（Au/Fc@CuO SPs）作为信号标记物，石墨烯负载中空碳球（HCNs-GR）作为基底。CuO SPs 具有超粒子结构，具有各向同性和有前途的催化活性的协同特性。二茂铁（Fc）沉积在 CuO SPs 上，作为电子传输介质。Au/Fc@CuO SPs 在提高免疫传感器的灵敏度方面发挥了关键作用。中空碳球（HCNs）负载的石墨烯（HCNs-GR）用于修饰电极表面。中空碳纳米球（HCNs）的嵌入减少了石墨烯纳米管堆积引起的有效表面积的减少。同时，碳球的负载进一步增加了石墨烯的表面积，使 HCNs-GR 能够更有效地固定抗体，提高了免疫传感器的灵敏度。所提出的免疫传感器在 500 fg/mL 至 100 ng/mL 的线性范围内显示出线性范围，检测限为 25.7 fg/mL。
