Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Organic Chemistry Institute and Centre for Soft Nanoscience, Westfälische Wilhelms-Universität Münster, Corrensstrasse 40, 48149, Münster, Germany.
Talanta. 2021 Sep 1;232:122478. doi: 10.1016/j.talanta.2021.122478. Epub 2021 May 10.
A procedure for the solid-phase extraction of antibiotics (enoxacin, ofloxacin, norfloxacin, ciprofloxacin, and sparfloxacin) in water has been developed. The sorbent used is based on a poly(glycidyl-co-ethylene dimethacrylate) network, whose previously modified surface has been functionalized with γ-cyclodextrin through a click-chemistry reaction. The architecture of the material has been characterized by thermogravimetric analysis, N adsorption-desorption, Raman spectroscopy, confocal microscopy, and scanning electron microscopy, showing good capability to be used as a filler for extraction cartridges. The optimization of the extraction methodology shows good intra-day and inter-day repeatability of the extraction procedure, with coefficients of variation between 2.5 and 5.1% and the possibility of reusing the material at least five times. The detection limits of the method have been established at the μg L level, confirming the possibility of quantifying trace levels. To end, real groundwater samples have been analyzed and the results are comparable with those obtained with a reference method. The proposed material can be used for assessing the presence of antibiotics in aqueous environments through an extraction procedure taking advantage of the presence of γ-cyclodextrin on its structure.
已经开发出一种用于水相中抗生素（依诺沙星、氧氟沙星、诺氟沙星、环丙沙星和司帕沙星）的固相萃取方法。所用的吸附剂基于聚（缩水甘油基-co-乙二甲基丙烯酸酯）网络，其先前修饰的表面通过点击化学反应与 γ-环糊精官能化。通过热重分析、N 吸附-解吸、拉曼光谱、共焦显微镜和扫描电子显微镜对材料的结构进行了表征，表明其具有作为萃取柱填料的良好性能。萃取方法的优化表明萃取过程具有良好的日内和日间重复性，变异系数在 2.5%至 5.1%之间，并且该材料至少可以重复使用五次。该方法的检测限已在μg L 水平确定，证实了定量痕量水平的可能性。最后，对实际地下水样品进行了分析，结果与参考方法获得的结果相当。通过利用其结构上存在的 γ-环糊精的萃取程序，该提出的材料可用于评估水环境中抗生素的存在。
