School of Agriculture, Ningxia University, Helan Mountain West Road 489, Hui Autonomous Region, Yinchuan, 750021, Ningxia, China.
Pingjibao Dairy Farm, Ningxia Agricultural Reclamation Helanshan Dairy Co. Ltd, Xixia District, Hui Autonomous Region, Yinchuan, 750021, Ningxia, China.
Sci Rep. 2021 Jun 1;11(1):11530. doi: 10.1038/s41598-021-91101-w.
ulked Segregant Analysis (BSA) is a rapid strategy for identifying genetic markers in specific regions of the phenotypical population and it has been widely used for QTLs mapping in smaller mixed F2 and F3 populations. We applied a modified BSA method to assessed genome-wide homozygous and heterozygous linkage patterns in the Chinese Wagyu Beef Cattle F2/F3 mixed population. Two overlapped regions from F2 and F3 populations on autosomes were found with high-density heterozygote alleles between high and low intramuscular fat groups. Regions from 24.8 M ~ 29.6 M of chromosome 23 were identified as most significantly correlated to the intramuscular fat in our samples. We also identified other 4 potential loci on chromosomes 5, 9, 15, and 21 correlated with Intramuscular fat. This study provided a novel low-cost method for QTLs mapping and identify molecular markers of phenotypical changes in a small mixed population.
连锁分离分析（BSA）是一种快速鉴定表型群体特定区域遗传标记的策略，已广泛用于较小的混合 F2 和 F3 群体中的 QTL 作图。我们应用改良的 BSA 方法评估了中国和牛 F2/F3 混合群体的全基因组纯合和杂合连锁模式。在 F2 和 F3 群体的两条常染色体重叠区域中，在高肌内脂肪和低肌内脂肪组之间发现了高密度杂合等位基因。在我们的样本中，23 号染色体 24.8 M 至 29.6 M 区域与肌内脂肪的相关性最为显著。我们还在 5、9、15 和 21 号染色体上鉴定到了与肌内脂肪相关的其他 4 个潜在位点。本研究为小混合群体的 QTL 作图和鉴定表型变化的分子标记提供了一种新的低成本方法。
