Departamento de Medicina Veterinária, Setor de Ciências Agrárias, Universidade Federal do Paraná - UFPR, Curitiba, PR, Brasil.
Programa de Pós-graduação em Ciências Veterinárias, Departamento de Medicina Veterinária, Setor de Ciências Agrárias, Universidade Federal do Paraná - UFPR, Curitiba, PR, Brasil.
Rev Bras Parasitol Vet. 2021 May 28;30(2):e001821. doi: 10.1590/S1984-29612021045. eCollection 2021.
Between December 2016 and April 2017, a spate of abortions occurred in a closed dairy herd from the central eastern region of Paraná, Brazil, in which 75 cows aborted. To identify its cause, organ fragments were collected from an aborted fetus for histopathology, and the blood samples from a stillborn, 4 aborted fetuses, and 9 farm dogs for indirect fluorescent antibody technique (IFAT). These tests found multifocal non-suppurative encephalitis, periportal hepatitis, and multifocal lymphoplasmacytic myocarditis, and detected anti-Neospora antibodies in all aborted fetuses, and in 5 of the 9 dogs. DNA of Neospora caninum was detected in the brain tissue of an aborted fetus. Blood samples of 340 cows and 146 heifers showed 33.5% and 30.8% seropositivity, respectively. In this closed herd, the parasite was probably introduced by infected domesticated or wild carnivores inhabiting the farm, through the infective oocysts present in their stool.
2016 年 12 月至 2017 年 4 月期间，巴西巴拉那州中东部一个封闭奶牛场发生了一系列流产事件，共有 75 头奶牛流产。为了确定病因，从流产胎儿中采集器官碎片进行组织病理学检查，并从死产牛、4 头流产胎儿和 9 条农场犬采集血液样本进行间接荧光抗体技术（IFAT）检测。这些检测发现了局灶性非化脓性脑炎、门脉周围肝炎和局灶性淋巴浆细胞性心肌炎，并在所有流产胎儿和 9 条犬中的 5 条中检测到抗新孢子虫抗体。在一头流产胎儿的脑组织中检测到了刚地弓形虫的 DNA。340 头奶牛和 146 头小母牛的血液样本分别显示出 33.5%和 30.8%的血清阳性率。在这个封闭的牛群中，寄生虫可能是通过栖息在农场的受感染的家养或野生食肉动物，通过其粪便中存在的感染性卵囊传播的。
