Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
Faculty of Kinesiology and Recreation Management, Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada.
Braz J Med Biol Res. 2021 May 31;54(9):e10700. doi: 10.1590/1414-431X2021e10700. eCollection 2021.
It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.
先前的研究表明，黑面神甲醇提取物（MFSOL）具有抗炎和促进创伤愈合的作用。因此，本研究探讨了 MFSOL 对人角质形成细胞（HaCaT）和实验性烧伤模型损伤的愈合作用。采用 HaCaT 细胞研究 MFSOL 对细胞迁移和增殖率的影响。雌性瑞士小鼠采用二度浅度烧伤模型，并分为 4 个治疗组：赋形剂组、1.0%磺胺嘧啶银组、0.5%和 1.0% MFSOL 乳膏（CrMFSOL）组。收集样本以定量评估炎症介质，在 3、7 和 14 天后进行组织学分析。结果表明，MFSOL（50μg/ml）通过增加增殖和迁移率来刺激 HaCaT 细胞。此外，0.5%CrMFSOL 可抑制髓过氧化物酶（MPO）活性，并在治疗后 3 天刺激白细胞介素（IL）-1β和 IL-10 的释放。CrMFSOL（0.5%）还能促进创面收缩，改善组织重塑，在第 7 天增加胶原生成，在第 14 天增加 VEGF 释放。因此，MFSOL 通过调节烧伤损伤的炎症介质，刺激人角质形成细胞（HaCaT）并促进创面愈合。
