Department of Hand Surgery, Yantaishan Hospital, Yantai, China.
Department of Clinical Laboratory, Yantaishan Hospital, Yantai, China.
Braz J Med Biol Res. 2021 May 31;54(9):e10842. doi: 10.1590/1414-431X2020e10842. eCollection 2021.
Regeneration of injured peripheral nerves is an extremely complex process. Nogo-A (neurite outgrowth inhibitor-A) inhibits axonal regeneration by interacting with Nogo receptor in the myelin sheath of the central nervous system (CNS). The aim of this study was to investigate the effects of Nogo-A and its receptor on the repair of sciatic nerve injury in rats. Sprague-Dawley rats (n=96) were randomly divided into 4 groups: control group (control), sciatic nerve transection group (model), immediate repair group (immediate repair), and delayed repair group (delayed repair). The rats were euthanized 1 week and 6 weeks after operation. The injured end tissues of the spinal cord and sciatic nerve were obtained. The protein expressions of Nogo-A and Nogo-66 receptor (NgR) were detected by immunohistochemistry. The protein expressions of Nogo-A, NgR, and Ras homolog family member A (RhoA) were detected by western blot. At 1 week after operation, the pathological changes in the immediate repaired group were less, and the protein expressions of Nogo-A, NgR, and RhoA in the spinal cord and sciatic nerve tissues were decreased (P<0.05) compared with the model group. After 6 weeks, the pathological changes in the immediate repair group and the delayed repair group were alleviated and the protein expressions decreased (P<0.05). The situation of the immediate repair group was better than that of the delayed repair group. Our data suggest that the expression of Nogo-A and its receptor increased after sciatic nerve injury, indicating that Nogo-A and its receptor play an inhibitory role in the repair process of sciatic nerve injury in rats.
损伤周围神经的再生是一个极其复杂的过程。Nogo-A（神经生长抑制因子-A）通过与中枢神经系统（CNS）髓鞘中的 Nogo 受体相互作用来抑制轴突再生。本研究旨在探讨 Nogo-A 及其受体对大鼠坐骨神经损伤修复的影响。Sprague-Dawley 大鼠（n=96）随机分为 4 组：对照组（对照）、坐骨神经切断组（模型）、即刻修复组（即刻修复）和延迟修复组（延迟修复）。术后 1 周和 6 周处死大鼠，获取脊髓和坐骨神经损伤末端组织。免疫组织化学法检测 Nogo-A 和 Nogo-66 受体（NgR）的蛋白表达。Western blot 法检测 Nogo-A、NgR 和 Ras 同源家族成员 A（RhoA）的蛋白表达。术后 1 周，即刻修复组的病理变化较轻，脊髓和坐骨神经组织中 Nogo-A、NgR 和 RhoA 的蛋白表达降低（P<0.05），与模型组比较。6 周后，即刻修复组和延迟修复组的病理变化均有所缓解，蛋白表达降低（P<0.05）。即刻修复组的情况优于延迟修复组。我们的数据表明，坐骨神经损伤后 Nogo-A 及其受体的表达增加，表明 Nogo-A 及其受体在大鼠坐骨神经损伤修复过程中起抑制作用。
