Hou Cheng-Yao, Zhang Liu-Hui, Zhang Yuan-Hang, Cui Jian-Tao, Zhao Li, Zheng Lan-Lan, Chen Hong-Ying
Zhengzhou Major Pig Disease Prevention and Control Laboratory, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China.
College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, People's Republic of China.
Transbound Emerg Dis. 2022 Jul;69(4):1890-1901. doi: 10.1111/tbed.14172. Epub 2021 Jun 11.
Porcine circovirus 4 (PCV4), a novel circovirus, was first discovered in April 2019 in Hunan Province of China. At present, PCV4 infection has been detected in China and South Korea. However, until 2019, there was little information about its circulating status and genetic characteristics. To further clarify the origin and prevalence of PCV4, a total of 152 clinical samples collected from 49 different swine farms of 15 cities in Henan Province of China from 2011 to 2021 were tested for the presence of PCV4 by qPCR, and the complete genome of PCV4 strains was amplified from the positive samples and sequenced. Among these samples, 45.39% (69/152) were positive for PCV4 and 86.67% (13/15) of the cities and 67.35% (33/49) of the swine farms were positive for PCV4. The genome sequences of 15 PCV4 strains were obtained, of which two PCV4 strains (HN-ZMD-201212 and HN-XX-201212) were achieved from archival samples in 2012, indicating that PCV4 has been circulating for at least 10 years in Henan Province of China. The phylogenetic analysis showed that 15 PCV4 strains in our study together with PCV4 strain HNU-AHG1-2019 were clustered into an identical but separate evolutionary branch, with genomic identity ranging from 98.2% to 98.8%. Our research further provides significant epidemiological information on PCV4 in China, which will help understand the origin and genetic characteristics of this new virus.
猪圆环病毒4型（PCV4）是一种新型圆环病毒，于2019年4月首次在中国湖南省被发现。目前，在中国和韩国均检测到了PCV4感染。然而，直到2019年，关于其流行状况和遗传特征的信息还很少。为了进一步阐明PCV4的起源和流行情况，对2011年至2021年从中国河南省15个城市的49个不同猪场采集的152份临床样本进行了qPCR检测，以确定是否存在PCV4，并从阳性样本中扩增出PCV4毒株的全基因组并进行测序。在这些样本中，45.39%（69/152）的样本PCV4呈阳性，15个城市中的86.67%（13/15）以及49个猪场中的67.35%（33/49）的PCV4呈阳性。获得了15株PCV4毒株的基因组序列，其中两株PCV4毒株（HN-ZMD-201212和HN-XX-201212）是从2012年的存档样本中获得的，这表明PCV4在中国河南省至少已经传播了10年。系统发育分析表明，我们研究中的15株PCV4毒株与PCV4毒株HNU-AHG1-2019聚集在一个相同但独立的进化分支中，基因组同一性范围为98.2%至98.8%。我们的研究进一步提供了关于中国PCV4的重要流行病学信息，这将有助于了解这种新病毒的起源和遗传特征。
