Zhengzhou Key Laboratory for Pig Disease Prevention and Control, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
Key Laboratory of "Runliang" Antiviral Medicines Research and Development, Institute of Drug Discovery & Development, Zhengzhou University, Zhengzhou, China.
Transbound Emerg Dis. 2021 Mar;68(2):276-282. doi: 10.1111/tbed.13714. Epub 2020 Jul 19.
Porcine circovirus 4 (PCV4), a new circovirus with a distinct relationship to other circoviruses, was identified in 2019 in several pigs with severe clinical disease in Hunan Province, China. To investigate the epidemic profile and genetic diversity of the virus, 63 clinical samples were collected from 24 different pig farms in 14 cities in Henan and Shanxi Provinces, China, between February 2018 and December 2019, and the partial Cap gene of PCV4 was amplified by PCR. Among the 63 samples, 16 (25.40%) were positive for PCV4, and 50% (12/24) of the pig farms were positive for PCV4. PCV4 was detected in samples from pigs with different clinical presentations. One PCV4 strain (Henan-LY1-2019) was sequenced in this study, and shared 98.4% genomic nucleotide identity with PCV4 strain HNU-AHG1-2019 (accession no. MK986820) detected on a pig farm in Hunan Province in 2019. A phylogenetic analysis based on the genomes of Henan-LY1-2019 and 31 reference strains showed that the Henan-LY1-2019 strain together with PCV4 strain HNU-AHG1-2019 was grouped in a relatively independent sub-branch, and separated from other viruses in the genus Circovirus. The results of this study extend our understanding of the molecular epidemiology of PCV4.
猪圆环病毒 4 型（PCV4）是一种新型圆环病毒，与其他圆环病毒具有明显的亲缘关系，于 2019 年在中国湖南省出现严重临床疾病的几头猪中被鉴定出来。为了调查该病毒的流行情况和遗传多样性，我们于 2018 年 2 月至 2019 年 12 月期间从中国河南省和山西省的 14 个城市的 24 个不同猪场采集了 63 份临床样本，并通过 PCR 扩增了 PCV4 的部分 Cap 基因。在 63 个样本中，有 16 个（25.40%）为 PCV4 阳性，其中 50%（12/24）的猪场为 PCV4 阳性。PCV4 在具有不同临床表现的猪的样本中均有检测到。本研究对一株 PCV4 分离株（河南-LY1-2019）进行了测序，该分离株与 2019 年在中国湖南省一个猪场检测到的 PCV4 株 HNU-AHG1-2019（登录号 MK986820）的基因组核苷酸序列同源性为 98.4%。基于河南-LY1-2019 株和 31 株参考株的基因组构建的系统进化树分析表明，河南-LY1-2019 株与 PCV4 株 HNU-AHG1-2019 一起被聚类在一个相对独立的分支中，与圆环病毒属中的其他病毒分离。本研究结果扩展了我们对 PCV4 分子流行病学的认识。
