Department of Biotechnology, University of Engineering and Management, Kolkata, India.
Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Kolkata, India.
J Appl Microbiol. 2021 Dec;131(6):2821-2837. doi: 10.1111/jam.15171. Epub 2021 Jun 22.
The aim of this study is to determine the ability of two bioactive compounds, namely, eugenol and linalool, purified from leaves of Ocimum tenuiflorum for eradication of biofilm produced by Pseudomonas aeruginosa.
The phytoextract of O. tenuiflorum (KT), a common ethno-botanical plant of India, was purified through high-performance liquid chromatography and was analysed using ultraviolet (UV) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Eugenol and linalool were found to be the most active amongst all phytocompounds present in phytoextract and showed a significant reduction in the viability of sessile cells of P. aeruginosa and the minimum revival after withdrawal of phyto-challenge. They could bring about notable reduction in the protein and carbohydrate content of exopolysaccharide of biofilm. Eugenol and linalool could affect the synthesis of quorum sensing (QS) proteins like LasA and LasB as well as virulence factors such as pyocyanin, and rhamnolipids, which seriously hamper the formation of biofilm. The biofilm framework was extremely affected by the phytocompounds through the reduction of protein and carbohydrate content of extracellular polymeric substance (EPS). Another interesting found out was that they brought about maximum inhibition to the genomic DNA and RNA content. The studies were supported by in silico interaction between eugenol and linalool with the QS proteins. The antibiofilm efficacies of eugenol, linalool and phytoextract (KT) were further confirmed by microscopic studies with scanning electron microscopy (SEM), atomic force microscopy and fluorescence confocal microscopy microscopic studies.
The phytocompounds are proved to be more effective than conventional antibiotics in inhibiting the biofilm forming sessile cells and can be used as a replacement for antibiotic.
Pure eugenol extracted from common basil leaves can be used as a safe substitute for common antibiotic for treatment of chronic infections caused by P. aeruginosa. It will be cost effective, devoid of notable side effects and will not generate antibiotic resistance in host body.
本研究旨在确定两种生物活性化合物，即丁香酚和芳樟醇，从罗勒叶中纯化，以消除铜绿假单胞菌生物膜的形成。
印度常见的民族植物药罗勒（KT）的植物提取物通过高效液相色谱法进行纯化，并通过紫外（UV）光谱法和气相色谱-质谱联用（GC-MS）进行分析。丁香酚和芳樟醇是植物提取物中最有效的植物化合物之一，对铜绿假单胞菌的静止细胞活力有显著降低作用，并且在植物挑战撤出后复活最小。它们可以显著降低生物膜外多糖的蛋白质和碳水化合物含量。丁香酚和芳樟醇可以影响群体感应（QS）蛋白如LasA 和 LasB 以及毒力因子如绿脓菌素和鼠李糖脂的合成，这严重阻碍了生物膜的形成。植物化合物通过降低细胞外聚合物（EPS）的蛋白质和碳水化合物含量，对生物膜结构产生了极大的影响。另一个有趣的发现是，它们对基因组 DNA 和 RNA 含量的抑制作用最大。这些研究得到了丁香酚和芳樟醇与 QS 蛋白之间的计算机模拟相互作用的支持。通过扫描电子显微镜（SEM）、原子力显微镜和荧光共聚焦显微镜微观研究进一步证实了丁香酚、芳樟醇和植物提取物（KT）的抗生物膜功效。
植物化合物在抑制形成生物膜的静止细胞方面比传统抗生素更有效，可以作为抗生素的替代品。
从常见罗勒叶中提取的纯丁香酚可作为治疗铜绿假单胞菌引起的慢性感染的安全替代常规抗生素。它将具有成本效益，没有显著的副作用，并且不会在宿主体内产生抗生素耐药性。
