School of Materials Science and Engineering, Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic Materials, Shaanxi University of Science and Technology, Xi'an 710021, PR China; College of Chemistry and Chemical Engineering, Yan'an University, Yan'an 716000, PR China.
School of Materials Science and Engineering, Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic Materials, Shaanxi University of Science and Technology, Xi'an 710021, PR China.
J Colloid Interface Sci. 2021 Nov;601:50-59. doi: 10.1016/j.jcis.2021.05.069. Epub 2021 May 18.
In this article, the dual-functional chicken egg white-copper phosphate organic-inorganic hybrid nanoflowers (Cu-NFs), combining the functions of signal amplification and biological recognition, were prepared through a simple one-pot method. The Cu-NFs exhibit excellent biocatalytic activity of peroxidase and polyphenol oxidase. Besides, a biotin-labeled secondary antibody encapsulated Cu-NFs-2 (Cu-NFs-2@Biotin-NHS-Ab) capture probe was prepared by using the interaction between avidin in the egg white and biotin. Based upon this superiority, the as-prepared Cu-NFs-2 were used in labeled avidin-biotin enzyme-linked immunosorbent assay (Cu-NFs-2 based-LAB-ELISA) to construct a sensitive colorimetric biosensor for the ultrasensitive detection of carcinoembryonic antigen (CEA). Under weak alkaline (pH = 7.5) conditions, the as-developed colorimetric sensor displayed a wide linear range of 0.05-40 ng/mL with a detection limit of 3.52 pg/mL. Furthermore, this colorimetric sensor has been successfully applied to the detection of CEA in human serum samples. Therefore, the as-developed colorimetric sensor has broad application prospects in the field of medical diagnosis and portable detection.
在本文中，通过简单的一锅法制备了具有信号放大和生物识别功能的双功能鸡卵清白磷铜有机-无机杂化纳米花（Cu-NFs）。Cu-NFs 表现出优异的过氧化物酶和多酚氧化酶的生物催化活性。此外，通过卵清白蛋白中的亲和素与生物素之间的相互作用，制备了一种生物素标记的二次抗体包被的 Cu-NFs-2（Cu-NFs-2@Biotin-NHS-Ab）捕获探针。基于这一优势，将制备的 Cu-NFs-2 用于标记亲和素-生物素酶联免疫吸附测定（Cu-NFs-2 基-LAB-ELISA）中，构建了用于超灵敏检测癌胚抗原（CEA）的灵敏比色生物传感器。在弱碱性（pH=7.5）条件下，所开发的比色传感器具有 0.05-40ng/mL 的宽线性范围，检测限为 3.52pg/mL。此外，该比色传感器已成功应用于人血清样品中 CEA 的检测。因此，所开发的比色传感器在医学诊断和便携式检测领域具有广阔的应用前景。
