Tsai Hweiyan, Chang Kaiying, Lee Wanshing, Fuh C Bor
Department of Medical Applied Chemistry, Chung Shan Medical University, Taichung 402, Taiwan.
Department of Medical Education, Chung Shan Medical University Hospital, Taichung 402, Taiwan.
Nanomaterials (Basel). 2021 Dec 28;12(1):66. doi: 10.3390/nano12010066.
Fluorescent carbon dots with blue, green, and red emissions were rapidly prepared from modified pine needles through microwave irradiation in a one-pot reaction. The fluorescence intensity and emission versatility for a carbon source were experimentally optimized. The reaction times were under 10 min and the reaction temperatures were lower than 220 °C. Potential applications of magnetic fluorescence-linked immunoassays of carcinoembryonic antigen (CEA) and tumor necrosis factor-alpha (TNF-α) were presented. The detection limits for CEA and TNF-α (3.1 and 2.8 pg mL, respectively) are lower than those presented in other reports, whereas the linear ranges for CEA and TNF-α (9 pg mL to 18 ng mL and 8.5 pg mL to 17 ng mL, respectively) are wider than those presented in other reports. Magnetic immunoassays with fluorescent CD prepared from pine needles can enable rapid, sensitive, and selective detections for biochemical analysis.
通过在一锅反应中进行微波辐照，由改性松针快速制备出具有蓝色、绿色和红色发射的荧光碳点。对碳源的荧光强度和发射多样性进行了实验优化。反应时间在10分钟以内，反应温度低于220℃。介绍了癌胚抗原（CEA）和肿瘤坏死因子-α（TNF-α）的磁性荧光联免疫分析的潜在应用。CEA和TNF-α的检测限（分别为3.1和2.8 pg/mL）低于其他报告中的检测限，而CEA和TNF-α的线性范围（分别为9 pg/mL至18 ng/mL和8.5 pg/mL至17 ng/mL）比其他报告中的更宽。用松针制备的荧光碳点进行磁性免疫分析能够实现对生化分析的快速、灵敏和选择性检测。
