Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
Rev Bras Ginecol Obstet. 2021 May;43(5):351-356. doi: 10.1055/s-0041-1730285. Epub 2021 Jun 2.
Most prenatal screening programs for toxoplasmosis use immunoassays in serum samples of pregnant women. Few studies assess the accuracy of screening tests in dried blood spots, which are of easy collection, storage, and transportation. The goals of the present study are to determine the performance and evaluate the agreement between an immunoassay of dried blood spots and a reference test in the serum of pregnant women from a population-based prenatal screening program for toxoplasmosis in Brazil.
A cross-sectional study was performed to compare the immunoassays Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzyme-linked fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control Program.
The dried blood spot test was able to discriminate positive and negative results of pregnant women when compared with the reference test, with an accuracy of 98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM).
Dried blood samples are easy to collect, store, and transport, and they have a good performance, making this a promising method for prenatal toxoplasmosis screening programs in countries with continental dimensions, limited resources, and a high prevalence of toxoplasmosis, as is the case of Brazil.
大多数弓形虫病产前筛查计划使用孕妇血清样本中的免疫测定法。很少有研究评估干血斑筛查试验的准确性，而干血斑易于采集、储存和运输。本研究的目的是确定基于人群的巴西弓形虫病产前筛查计划中干血斑免疫测定法的性能，并评估其与血清参考检测方法的一致性。
进行了一项横断面研究，以比较 Imunoscreen Toxoplasmose IgM 和 Imunoscreen Toxoplasmose IgG（Mbiolog Diagnósticos，Ltda.，Contagem，Minas Gerais，巴西）在干血斑中的免疫测定法与酶联荧光测定法（ELFA，BioMérieux S.A.，Lyon，法国）在 Minas Gerais 先天性弓形虫病控制计划孕妇血清中的参考标准。
与参考检测方法相比，干血斑检测能够区分孕妇的阳性和阴性结果，其对免疫球蛋白 G（IgG）的准确性为 98.2%，对免疫球蛋白 M（IgM）的准确性为 95.8%。
干血样本易于采集、储存和运输，并且具有良好的性能，因此对于具有大陆规模、资源有限且弓形虫病流行率高的国家（如巴西）的产前弓形虫病筛查计划来说，这是一种很有前途的方法。
