Department of Chemistry, Colorado School of Mines, Golden, CO, 80401, USA.
Corporate Strategic Research, ExxonMobil, Annandale, NJ, 08801, USA.
Sci Rep. 2021 Jun 2;11(1):11649. doi: 10.1038/s41598-021-91106-5.
With fast growth rates, broad halotolerance and the ability to thrive at high temperatures, algae in the genus Picochlorum are emerging as promising biomass producers. Recently, we isolated a remarkably productive strain, Picochlorum celeri, that attains > 40 g m day productivities using simulated outdoor light. To test outdoor productivities, Picochlorum celeri was cultivated in 820 L raceway ponds at the Arizona Center for Algae Technology and Innovation. Picochlorum celeri demonstrated the highest outdoor biomass productivities reported to date at this testbed averaging ~ 31 g m day over four months with a monthly (August) high of ~ 36 g m day. Several single day productivities were > 40 g m day. Importantly for sustainability, Picochlorum celeri achieved these productivities in saline water ranging from seawater to 50 parts per thousand sea salts, without any biocides or pond crashes, for over 143 days. Lastly, we report robust genetic engineering tools for future strain improvements.
微绿球藻属的藻类具有快速的增长率、广泛的耐盐性和在高温下茁壮成长的能力，正逐渐成为有前途的生物量生产菌。最近，我们分离出一种生产力极高的菌株微绿球藻属的芹菜，该菌株在模拟户外光照下的产量超过 40 g m-2 day-1。为了测试户外生产力，微绿球藻属的芹菜在亚利桑那藻类技术和创新中心的 820 L 跑道池塘中进行了培养。微绿球藻属的芹菜在该试验基地的四个月中平均每天的生物量产量高达 31 g m-2 day-1，达到了迄今为止报告的最高户外生物量产量，其中 8 月份的单月产量最高，达到 36 g m-2 day-1。有几天的日产量超过 40 g m-2 day-1。重要的是，对于可持续性，微绿球藻属的芹菜在从海水到 50 ppt 海水盐度的盐水中实现了这些产量，没有使用任何杀菌剂或池塘崩溃，持续了超过 143 天。最后，我们报告了强大的遗传工程工具，用于未来的菌株改良。
