Removal of contaminating nucleoside diphosphates from commercial preparations of uridine diphosphoglucose.
作者信息
Outlaw W H, Hite D R, Fiore G B
机构信息
Department of Biological Science, Florida State University, Tallahassee 32306-3050.
出版信息
Anal Biochem. 1988 May 15;171(1):104-7. doi: 10.1016/0003-2697(88)90129-7.
PMID:3407906
Abstract
Nucleoside diphosphate contamination in commercial preparations of UDP-Glc poses potential problems in activity assays for enzymes that use this substrate. For removing these contaminants, we report a simple, inexpensive, and rapid method that obviates the need for uncertain corrections in assay calculations.
摘要
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