利用动物酶在体外形成尿苷二磷酸2-脱氧-D-葡萄糖和鸟苷二磷酸2-脱氧-D-葡萄糖。

Formation of uridine diphosphate 2-deoxy-D-glucose and guanosine diphosphate 2-deoxy-D-glucose in vitro using animal enzymes.

作者信息

Schwarz R T, Schmidt F G

出版信息

Eur J Biochem. 1976 Feb 2;62(1):181-7. doi: 10.1111/j.1432-1033.1976.tb10111.x.

DOI:10.1111/j.1432-1033.1976.tb10111.x

Abstract

The enzymic formation of guanosine diphosphate 2-deoxy-D-glucose and uridine diphosphate 2-deoxy-D-glucose from synthetically prepared 2-deoxy-D-glucose 1-phosphate is described. Incubation of 2-deoxy-D-glucose 1-phosphate with an enzyme preparation from bovine mammary glands and either GTP or UTP gives rise to the corresponding nucleoside-diphosphate derivative of 2-deoxy-D-glucose. Uridine diphosphate 2-deoxy-D-glucose could also be obtained by incubation of 2-deoxy-D-glucose 1-phosphate with UTP and UDP glucose pyrophosphorylase from beef liver.

摘要

本文描述了由人工合成制备的1-磷酸-2-脱氧-D-葡萄糖通过酶促反应生成二磷酸鸟苷-2-脱氧-D-葡萄糖和二磷酸尿苷-2-脱氧-D-葡萄糖的过程。将1-磷酸-2-脱氧-D-葡萄糖与牛乳腺的酶制剂以及鸟苷三磷酸（GTP）或尿苷三磷酸（UTP）一起温育，可生成相应的2-脱氧-D-葡萄糖核苷二磷酸衍生物。将1-磷酸-2-脱氧-D-葡萄糖与UTP以及来自牛肝的尿苷二磷酸葡萄糖焦磷酸化酶一起温育，也可得到二磷酸尿苷-2-脱氧-D-葡萄糖。