羟基自由基的比色检测：各种铁配合物产生羟基自由基能力的比较
Colorimetric detection of the hydroxyl radical: comparison of the hydroxyl-radical-generating ability of various iron complexes.
作者信息
Singh S, Hider R C
机构信息
Department of Chemistry, University of Essex, Colchester, United Kingdom.
出版信息
Anal Biochem. 1988 May 15;171(1):47-54. doi: 10.1016/0003-2697(88)90123-6.
PMID:3407920
Abstract
An aromatic substrate for hydroxylation by OH radicals has been synthesized. Neither the substrate nor its hydroxylated product binds either iron(III) or iron(II). A spectrophotometric assay based on the hydroxylation of this substrate, N,N'-(5-nitro-1,3-phenylene)bisglutaramide, is described.
摘要
已合成了一种用于被羟基自由基羟基化的芳香族底物。该底物及其羟基化产物均不与铁（III）或铁（II）结合。本文描述了一种基于该底物N，N'-（5-硝基-1,3-亚苯基）双戊二酰胺羟基化的分光光度测定法。
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