Institute of Pharmaceutical Science, King's College London, London SE1 9NH, UK.
Centre for Therapeutic Innovation, University of Bath, Bath BA2 7AY, UK.
Int J Mol Sci. 2023 Feb 19;24(4):4162. doi: 10.3390/ijms24044162.
An aromatic substrate for hydroxylation by hydroxyl radicals (OH) was investigated. The probe, '-(5-nitro-1,3-phenylene)--glutaramide, and its hydroxylated product do not bind either iron(III) or iron(II), and so they do not interfere with the Fenton reaction. A spectrophotometric assay based on the hydroxylation of the substrate was developed. The synthesis and purification methods of this probe from previously published methodologies were improved upon, as well as the analytical procedure for monitoring the Fenton reaction through its use, enabling univocal and sensitive OH detection. The assay was utilised to demonstrate that the iron(III) complexes of long-chain fatty acids lack Fenton activity under biological conditions.
研究了一种可被羟基自由基（OH）羟化的芳基底物。该探针为 '-(5-硝基-1,3-亚苯基)--谷氨酸酰胺'，其羟化产物既不与铁（III）也不与铁（II）结合，因此不会干扰芬顿反应。基于该底物的羟化作用，建立了分光光度测定法。该探针的合成和纯化方法是在先前发表的方法的基础上进行改进的，以及通过使用该探针监测芬顿反应的分析程序，实现了对 OH 的明确和敏感检测。该测定法用于证明在生物条件下，长链脂肪酸的铁（III）配合物没有芬顿活性。
