Department of Pulmonology, 2nd Department of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Kraków, Poland.
2nd Department of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Kraków, Poland.
Int J Chron Obstruct Pulmon Dis. 2021 May 25;16:1415-1424. doi: 10.2147/COPD.S298678. eCollection 2021.
Lipid mediators, particularly eicosanoids, are associated with airway inflammation, especially with the eosinophilic influx. This study aimed to measure lipid mediators and cells in induced sputum, that could possibly reflect the inflammatory process in the bronchial tree of COPD subjects.
Eighty patients diagnosed with COPD and 37 healthy controls participated in the study. Induced sputum samples were ascertained for differential cell count and induced sputum supernatant concentrations of selected eicosanoids by the means of gas chromatography/mass spectrometry and high-performance liquid chromatography/tandem mass spectrometry.
Increased sputum eosinophilia was associated with higher concentrations of selected proinflammatory eicosanoids. In COPD subjects prostaglandin D and 11-dehydro-thromboxane B correlated negatively with airway obstruction measured by FEV and FEV/FVC values. COPD subjects with disease exacerbations during past 12 months had significantly higher concentrations of prostaglandin D, 12-oxo-eicosatetraenoic acid and 5-oxo-eicosatetraenoic acid.
Stable COPD is often associated with eosinophil influx in the lower airways and elevated concentrations of eicosanoids that is reflected by some disease characteristics.
脂质介质，特别是类二十烷酸，与气道炎症有关，特别是与嗜酸性粒细胞浸润有关。本研究旨在测量诱导痰中的脂质介质和细胞，这些介质和细胞可能反映 COPD 患者支气管树中的炎症过程。
80 名被诊断为 COPD 的患者和 37 名健康对照者参与了这项研究。通过气相色谱/质谱和高效液相色谱/串联质谱法，确定诱导痰样本中的差异细胞计数和选定类二十烷酸的诱导痰上清液浓度。
痰中嗜酸性粒细胞增多与选定的促炎类二十烷酸浓度升高有关。在 COPD 患者中，前列腺素 D 和 11-脱氢血栓烷 B 与 FEV 和 FEV/FVC 值所测气道阻塞呈负相关。在过去 12 个月中疾病加重的 COPD 患者中，前列腺素 D、12-氧代-二十碳四烯酸和 5-氧代-二十碳四烯酸的浓度显著升高。
稳定的 COPD 常与下气道嗜酸性粒细胞浸润和某些疾病特征反映的类二十烷酸浓度升高有关。
