Gorska Katarzyna, Nejman-Gryz Patrycja, Paplinska-Goryca Magdalena, Korczynski Piotr, Prochorec-Sobieszek Monika, Krenke Rafal
a Department of Internal Medicine, Pulmonary Diseases and Allergy , Medical University of Warsaw , Warsaw , Poland.
b Department of Pathology , Institute of Rheumatology , Warsaw , Poland.
COPD. 2018 Feb;15(1):36-45. doi: 10.1080/15412555.2017.1416074. Epub 2018 Jan 10.
IL-6 and IL-33 are involved in the inflammatory process in obstructive lung diseases. In contrast to IL-6, few data on the expression of IL-33 in different biological samples from asthma and COPD patients are available. The aim was to evaluate the expressions of IL-33 and IL-6 in bronchial mucosa and to compare these expressions with the concentrations of both cytokines in various respiratory samples from patients with mild-to-moderate asthma and COPD. Serum, induced sputum and exhaled breath condensate IL-6 and IL-33 levels, as well as their expression in bronchial mucosa were evaluated in 22 asthma and 33 COPD patients. There were significant differences between bronchial mucosa IL-6, but not IL-33 expression in asthma and COPD. Serum and IS IL-6 concentrations were higher in COPD than in asthma (3.4 vs. 2.02 pg/mL, p = 0.002 and 16.5 vs. 12.7 pg/mL, p = 0.007, respectively); IL-33 levels reached similar values in asthma and COPD in all investigated samples. In both diseases, the lowest levels of IL-6 and IL-33 were found in EBC. EBC levels of both cytokines did not correlate with their expression in other materials. The IL-33 and IL-6 are detectable in serum, IS and EBC not only in asthma but also in COPD patients. In the COPD group, serum and IS IL-6 concentrations were statistically higher than in the asthma group. The tissue expression of IL-33 and IL-33 concentrations in the investigated biological samples were on a comparable level in both diseases. Our findings may suggest that IL-33 activation is a common pathway in asthma and COPD.
白细胞介素-6（IL-6）和白细胞介素-33（IL-33）参与阻塞性肺疾病的炎症过程。与IL-6不同，关于哮喘和慢性阻塞性肺疾病（COPD）患者不同生物样本中IL-33表达的数据很少。目的是评估支气管黏膜中IL-33和IL-6的表达，并将这些表达与轻至中度哮喘和COPD患者各种呼吸样本中两种细胞因子的浓度进行比较。对22例哮喘患者和33例COPD患者的血清、诱导痰和呼出气冷凝液中的IL-6和IL-33水平及其在支气管黏膜中的表达进行了评估。哮喘和COPD患者支气管黏膜IL-6表达存在显著差异，但IL-33表达无显著差异。COPD患者血清和诱导痰中IL-6浓度高于哮喘患者（分别为3.4 vs. 2.02 pg/mL，p = 0.002和16.5 vs. 12.7 pg/mL，p = 0.007）；在所有研究样本中，哮喘和COPD患者的IL-33水平相似。在两种疾病中，呼出气冷凝液中IL-6和IL-33水平最低。两种细胞因子的呼出气冷凝液水平与其在其他样本中的表达无相关性。IL-33和IL-6不仅在哮喘患者中，而且在COPD患者的血清、诱导痰和呼出气冷凝液中均可检测到。在COPD组中，血清和诱导痰中IL-6浓度在统计学上高于哮喘组。两种疾病中研究的生物样本中IL-33的组织表达和IL-33浓度处于可比水平。我们的研究结果可能表明IL-33激活是哮喘和COPD的共同途径。
