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叶片提取物和绿色合成银纳米粒子的体外抗癌作用。

In vitro Anticancer Effects of Leaf Extract and Green-Synthesised Silver Nanoparticles.

机构信息

Integrative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Sains@BERTAM, Universiti Sains Malaysia, Kepala Batas, 13200, Pulau Pinang, Malaysia.

Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Sains@BERTAM, Universiti Sains Malaysia, Kepala Batas, 13200, Pulau Pinang, Malaysia.

出版信息

Int J Nanomedicine. 2021 May 25;16:3599-3612. doi: 10.2147/IJN.S303921. eCollection 2021.

DOI:10.2147/IJN.S303921
PMID:34079252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8164701/
Abstract

PURPOSE

(VA) is a traditional African herbal medicine that has been reported to possess anticancer properties. However, the anticancer properties of VA silver nanoparticles have not been studied. The aim of the study was to examine and evaluate the anticancer activities of VA leaf extracts and VA silver nanoparticles on the human breast cancer cell line, MCF-7.

METHODS

VA leaves were extracted using sequential extraction assisted with ultrasound using three different solvents: ethanol, 50% ethanol, and deionized water. The silver nanoparticles were synthesised with VA aqueous extract.

RESULTS

The ethanol extract and VA silver nanoparticles inhibit MCF-7 cell proliferation with an average half-maximal inhibitory concentration (IC) value of 67µg/mL and 6.11µg/mL, respectively, after 72 hours of treatment. The ethanol extract and VA silver nanoparticles also caused G1 phase cell cycle arrest, induced apoptosis and nuclear fragmentation in MCF-7 cells.

CONCLUSION

VA ethanol extracts and VA silver nanoparticles decreased the cell viability in MCF-7 cells in a time and dose-dependent manner by inducing apoptosis and causing DNA damage. Further research is needed to elucidate the mechanism of action of VA leaf extracts and VA silver nanoparticles. This study is the first to report on the anticancer activity of VA silver nanoparticles in MCF-7 cells.

摘要

目的

（VA）是一种传统的非洲草药，据报道具有抗癌特性。然而，VA 银纳米粒子的抗癌特性尚未得到研究。本研究旨在研究和评估 VA 叶提取物和 VA 银纳米粒子对人乳腺癌细胞系 MCF-7 的抗癌活性。

方法

使用超声辅助的连续提取法，用三种不同的溶剂：乙醇、50%乙醇和去离子水从 VA 叶中提取。用 VA 水提取物合成银纳米粒子。

结果

乙醇提取物和 VA 银纳米粒子在 72 小时的处理后，分别以平均半最大抑制浓度（IC）值 67μg/mL 和 6.11μg/mL 抑制 MCF-7 细胞增殖。乙醇提取物和 VA 银纳米粒子还导致 MCF-7 细胞的 G1 期细胞周期停滞，诱导细胞凋亡和核碎裂。

结论

VA 乙醇提取物和 VA 银纳米粒子通过诱导细胞凋亡和造成 DNA 损伤，以时间和剂量依赖的方式降低 MCF-7 细胞的细胞活力。需要进一步研究来阐明 VA 叶提取物和 VA 银纳米粒子的作用机制。本研究首次报道了 VA 银纳米粒子在 MCF-7 细胞中的抗癌活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/8f685ec732b5/IJN-16-3599-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/fc4c716fdfb5/IJN-16-3599-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/bbe91f0d8cdd/IJN-16-3599-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/8055d2baaa39/IJN-16-3599-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/6283fb6444b1/IJN-16-3599-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/b2682a193c7b/IJN-16-3599-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/f7f23f1833f2/IJN-16-3599-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/b502391df804/IJN-16-3599-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/07b4dca9c820/IJN-16-3599-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/8f685ec732b5/IJN-16-3599-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/fc4c716fdfb5/IJN-16-3599-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/bbe91f0d8cdd/IJN-16-3599-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/8055d2baaa39/IJN-16-3599-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/6283fb6444b1/IJN-16-3599-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/b2682a193c7b/IJN-16-3599-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/f7f23f1833f2/IJN-16-3599-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/b502391df804/IJN-16-3599-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/07b4dca9c820/IJN-16-3599-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bfb/8164701/8f685ec732b5/IJN-16-3599-g0009.jpg

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