Department of Otorhinolaryngology, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.
Department of Operating Room, Weifang Yidu Central Hospital, Weifang, Shandong 262500, P.R. China.
Mol Med Rep. 2021 Jul;24(1). doi: 10.3892/mmr.2021.12173. Epub 2021 Jun 3.
Nasopharyngeal carcinoma (NPC) is a common malignant tumor in South China and is characterized by a high death rate. Ophiopogonin B (OP‑B) is a bioactive component of Radix , which is frequently used in traditional Chinese medicine to treat cancer. The present study aimed to examine the anti‑cancer properties of OP‑B on NPC cells. Cell viability and cell proliferation were measured using MTT and EdU assays. Flow cytometry was used to measure cell apoptosis, reactive oxygen species and mitochondrial membrane potential. Western blotting was used to investigate the expression of apoptosis and Hippo signaling pathway proteins. OP‑B inhibited the proliferation of NPC cells by inducing apoptosis and disturbing the mitochondrial integrity. OP‑B enhanced ROS accumulation. In addition, OP‑B promoted the expression of mammalian STE20‑like kinase 1, large tumor suppressor 1 and phosphorylated yes‑associated protein (YAP) and suppressed the expression of YAP and transcriptional enhanced associate domain in NPC cells. OP‑B increased the expression of forkhead box transcription factor O1 in the nuclear fraction. In conclusion, OP‑B has therapeutic potential and feasibility in the development of novel YAP inhibitors for NPC.
鼻咽癌（NPC）是华南地区常见的恶性肿瘤，其死亡率较高。麦冬皂苷 B（OP-B）是中药麦冬的一种生物活性成分，常用于治疗癌症。本研究旨在探讨 OP-B 对 NPC 细胞的抗癌特性。通过 MTT 和 EdU 检测法来测量细胞活力和增殖。通过流式细胞术来测量细胞凋亡、活性氧和线粒体膜电位。通过 Western blot 法来研究凋亡和 Hippo 信号通路蛋白的表达。OP-B 通过诱导细胞凋亡和破坏线粒体完整性来抑制 NPC 细胞的增殖。OP-B 促进 ROS 积累。此外，OP-B 促进哺乳动物 STE20 样激酶 1、大肿瘤抑制因子 1 和磷酸化 YAP 的表达，并抑制 NPC 细胞中 YAP 和转录增强相关域的表达。OP-B 增加了核部分 forkhead box 转录因子 O1 的表达。总之，OP-B 在开发 NPC 的新型 YAP 抑制剂方面具有治疗潜力和可行性。
