哥伦比亚蜂胶样品在犬骨肉瘤细胞系中诱导的凋亡相关基因表达
Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line.
作者信息
Pardo-Mora Dolly Patricia, Murillo Oscar Julián, Rey-Buitrago Mauricio, Losada-Barragán Mónica, Uribe Jaime Fabian Cruz, Santiago Karina Basso, Conti Bruno José, Cardoso Eliza de Oliveira, Conte Fernanda Lopes, Gutiérrez Rafael María, García Orlando Torres, Sforcin José Maurício
机构信息
Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.
Department of Morphology, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
出版信息
Vet World. 2021 Apr;14(4):964-971. doi: 10.14202/vetworld.2021.964-971. Epub 2021 Apr 22.
BACKGROUND AND AIM
Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of , , , , and genes involved in the apoptosis pathway.
MATERIALS AND METHODS
After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction.
RESULTS
The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated and expression. Usm and Sil increased expression, Met and Sil induced expression, and Usm increased .
CONCLUSION
Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and upregulation indicated apoptosis induction by the extrinsic pathway.
背景与目的
骨肉瘤（OSA）是犬类和人类中最常见的骨肿瘤。本研究旨在通过评估参与凋亡途径的、、、、和基因的表达，来评估哥伦比亚蜂胶样品对犬骨肉瘤细胞系（OSCA - 8）的细胞毒性和凋亡作用。
材料与方法
用五种哥伦比亚蜂胶样品（Usm、Met、Fus、Sil和Caj）处理细胞后，我们评估了细胞活力和乳酸脱氢酶（LDH）释放。使用膜联蛋白V/碘化丙啶通过流式细胞术测定早期和晚期凋亡。此外，通过实时聚合酶链反应分析了三种选定样品对基因表达的影响。
结果
哥伦比亚蜂胶样品降低了OSCA - 8细胞活力并增加了LDH释放。所有样品均显著诱导凋亡并上调和表达。Usm和Sil增加了表达，Met和Sil诱导了表达，Usm增加了。
结论
哥伦比亚蜂胶样品对犬骨肉瘤细胞表现出细胞毒性和凋亡作用，上调表明通过外源性途径诱导凋亡。
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