Ulutaş Pınar Alkım, Kıral Funda, Ulutaş Bülent, Aşıcı Gamze Sevri Ekren
Department of Biochemistry, Aydin, Turkey.
Department of Internal Medicine, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, 09010, Aydin, Turkey.
J Vet Res. 2020 Oct 8;64(4):589-596. doi: 10.2478/jvetres-2020-0063. eCollection 2020 Dec.
Clinoptilolite has antiviral, antibacterial, anti-inflammatory, antidiabetic, and anticancer properties due to its biological activities. In various cancer cell culture studies, it has been reported effective against tumour cells and gave positive results in treatment of various tumours in dogs. No study was found on the effects of the nanoparticulate form, nanoclinoptilolite, on cancer cells. The aim of this study was to determine its cytotoxic and apoptotic effects in canine osteosarcoma (OSA) cell culture.
Doses at 50% inhibitory concentration were determined by measuring the dose- and duration-dependent cytotoxicity of nanoclinoptilolite on canine D-17 osteosarcoma cells by methylthiazol tetrazolium (MTT) test for 24 h, 48 h, and 72 h. Murine caspase-3 and -7 activity and expression levels of the and genes were measured using RT-PCR to investigate the apoptotic effect.
Nanoclinoptilolite decreased cell viability and induced caspase-3- and -7-mediated apoptosis in treated canine OSA cells. Furthermore, its application to canine OSA cells downregulated the expression of and upregulated the expression of proapoptotic .
Clinoptilolite, which was previously demonstrated to have anticancer properties, decreased cell viability effectively and rapidly and increased the apoptotic cell ratio in a novel use in nanoparticle form, exhibiting this effect by increasing the ratio.
斜发沸石因其生物活性而具有抗病毒、抗菌、抗炎、抗糖尿病和抗癌特性。在各种癌细胞培养研究中，据报道它对肿瘤细胞有效，并在犬类各种肿瘤的治疗中取得了积极成果。尚未发现有关纳米颗粒形式的斜发沸石（纳米斜发沸石）对癌细胞影响的研究。本研究的目的是确定其在犬骨肉瘤（OSA）细胞培养中的细胞毒性和凋亡作用。
通过噻唑蓝（MTT）试验测量纳米斜发沸石对犬D - 17骨肉瘤细胞在24小时、48小时和72小时的剂量和时间依赖性细胞毒性，确定50%抑制浓度的剂量。使用逆转录聚合酶链反应（RT - PCR）测量小鼠半胱天冬酶 - 3和 - 7的活性以及 和 基因的表达水平，以研究凋亡作用。
纳米斜发沸石降低了处理后的犬OSA细胞的活力，并诱导了半胱天冬酶 - 3和 - 7介导的凋亡。此外，将其应用于犬OSA细胞下调了 的表达，并上调了促凋亡 的表达。
先前已证明具有抗癌特性的斜发沸石，以纳米颗粒形式的新用途有效地快速降低了细胞活力，并增加了凋亡细胞比例，通过增加 比例表现出这种效果。
