Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia.
Nutr Cancer. 2022;74(3):978-995. doi: 10.1080/01635581.2021.1931701. Epub 2021 Jun 4.
Clinical efficacy of chemotherapy is often compromised by diabetogenic glucose on colorectal cancer (CRC). High glucose has been shown to diminish the cytotoxicity of anticancer drugs. The issue can potentially be addressed with natural products. Recently, we revealed that exhibits inhibitory activities against human colonic carcinoma cells. In this study, the impacts of hexane fraction (Hex, sterol-enriched) and chloroform fraction (Chl, terpenoid-enriched) were further elucidated. The cellular responses, including oxidative stress, cell cycle, and apoptosis were compared between the presence of normal glucose (NG, 5.5 mM) and high glucose (HG, 25 mM). HG promoted cell viability with concomitant elevation of GSH level. Both Hex and Chl fractions stimulated NO production, in addition, induced cell cycle arrest. The apoptotic effect of Hex fraction was glucose-dependent, but Chl fraction triggered apoptosis with an equivalent extent in NG and HG conditions. Overall, the active fractions from show therapeutic potential in managing hyperglycemia-associated CRC.
化疗的临床疗效常因致糖尿病的葡萄糖而受到影响，这种葡萄糖会降低抗癌药物的细胞毒性。天然产物可能会解决这个问题。最近，我们发现 对人结肠癌细胞具有抑制活性。在这项研究中，进一步阐明了正己烷部分（富含甾醇）和氯仿部分（富含萜烯）的影响。在正常葡萄糖（NG，5.5mM）和高葡萄糖（HG，25mM）存在的情况下，比较了细胞反应，包括氧化应激、细胞周期和细胞凋亡。HG 促进细胞活力，同时提高 GSH 水平。正己烷部分和氯仿部分均能刺激 NO 产生，并诱导细胞周期停滞。正己烷部分的细胞凋亡作用依赖于葡萄糖，但氯仿部分在 NG 和 HG 条件下具有相同程度的诱导细胞凋亡作用。总的来说， 的活性部分在治疗与高血糖相关的 CRC 方面具有潜在的治疗作用。
