Tan Wee-Cheat, Kuppusamy Umah Rani, Phan Chia-Wei, Tan Yee-Shin, Raman Jegadeesh, Anuar Azliza Mad, Sabaratnam Vikineswary
1] Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia [2] Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
1] Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia [2] Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Sci Rep. 2015 Jul 27;5:12515. doi: 10.1038/srep12515.
Mushroom cultivation benefits humankind as it deliberately encourages wild mushrooms to be commercially propagated while recycling agricultural wastes. Ganoderma neo-japonicum is a rare polypore mushroom found growing on decaying Schizostachyum brachycladium (a tropical bamboo) clumps in Malaysia. The Malaysian indigenous tribes including the Temuans and Temiars use the basidiocarps of G. neo-japonicum to treat various ailments including diabetes. In this study, the domestication of G. neo-japonicum in artificial logs of different agricultural residues was investigated. Sawdust promoted the mycelia spawn colonisation in the shortest period of 38 ± 0.5 days. However, only sawdust and bamboo dust supported the primodia formation. Complex medium supported mycelium growth in submerged cultures and 27.11 ± 0.43 g/L of mycelia was obtained after 2 weeks of cultivation at 28 °C and 200 rpm. Antioxidant potential in mushroom may be influenced by different cultivation and extraction methods. The different extracts from the wild and cultivated basidiocarps as well as mycelia were then tested for their antioxidant properties. Aqueous and ethanol extracts of mycelia and basidiocarps tested had varying levels of antioxidant activities. To conclude, domestication of wild G. neo-japonicum using agroresidues may ensure a continuous supply of G. neo-japonicum for its medicinal use while ensuring the conservation of this rare species.
蘑菇栽培对人类有益，因为它有意促进野生蘑菇的商业繁殖，同时回收农业废弃物。新日本灵芝是一种罕见的多孔菌，生长在马来西亚腐烂的短穗竹丛上。包括 Temuans 和 Temiars 在内的马来西亚本土部落使用新日本灵芝的子实体来治疗包括糖尿病在内的各种疾病。在这项研究中，研究了在不同农业残留物的人工原木中对新日本灵芝进行驯化。锯末在最短的 38±0.5 天内促进了菌丝体菌种的定殖。然而，只有锯末和竹粉支持原基形成。复合培养基支持深层培养中的菌丝体生长，在 28°C 和 200 rpm 下培养 2 周后，获得了 27.11±0.43 g/L 的菌丝体。蘑菇中的抗氧化潜力可能受不同的栽培和提取方法影响。然后测试了野生和栽培子实体以及菌丝体的不同提取物的抗氧化性能。测试的菌丝体和子实体的水提取物和乙醇提取物具有不同水平的抗氧化活性。总之，利用农业残留物对野生新日本灵芝进行驯化可以确保其药用的持续供应，同时确保保护这种珍稀物种。
