School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.
School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Nov 15;261:120009. doi: 10.1016/j.saa.2021.120009. Epub 2021 May 26.
A fluorescent probe based on a glycyl-L-tyrosine-modified dansyl derivative (D-GT) is designed and synthesized. D-GT demonstrated great detection performance toward Cu in an aqueous solution. Fluorescence quenching occurred due to the coordination of Cu with D-GT. The sensitive detection of D-GT to Cu was applied in aqueous solution within a wide pH span (6-12). A 1:1 coordinate stoichiometric way and an association constant of 6.47 × 10 M between D-GT and Cu were determined. The measured detection limit for Cu in HEPES buffer solution (10 mM, pH 7.4) was 0.69 μM. The probe displayed an appropriate sensitivity toward Cu in real drinking water samples and living cells, which reveals the potential applications of D-GT in complicated environments.
基于甘氨酰-L-酪氨酸修饰的丹磺酰衍生物（D-GT）设计并合成了一种荧光探针。D-GT 在水溶液中对 Cu 表现出出色的检测性能。由于 Cu 与 D-GT 的配位，发生了荧光猝灭。D-GT 对 Cu 的灵敏检测可在宽 pH 范围（6-12）内应用于水溶液中。确定了 D-GT 与 Cu 之间以 1:1 配位计量比和 6.47×10 M 的结合常数。在 HEPES 缓冲溶液（10 mM，pH 7.4）中测定 Cu 的检测限为 0.69 μM。该探针对实际饮用水样品和活细胞中的 Cu 具有适当的灵敏度，这表明 D-GT 在复杂环境中具有潜在的应用。
