Berliner S, Fishelson Z, Wasserman L, Pinkhas J, Aronson M
Department of Internal Medicine D, Beilinson Medical Center, Rehovot, Israel.
Biomed Pharmacother. 1988;42(1):69-72.
During the course of extracorporeal circulation, leukocyte aggregation is known to occur in some patients. This is attributed to complement activation and release of the chemotactic factor C5a. We investigated the effect of heparin on in vitro complement induced aggregation of polymorphonuclear leukocytes (PMNAGG), since most patients undergo heparinization during the extracorporeal circulation. Our results indicate that sub-aggregating concentrations of zymosan-activated serum (ZAS) enhance heparin-induced PMNAGG and that sub-aggregating amounts of heparin increase the aggregation induced by ZAS. Heat inactivation of the serum prior to zymosan activation abrogated the aggregating activity of the ZAS. Furthermore, the combined ZAS and heparin-induced PMNAGG was partially inhibited by specific antibodies to C5a but not to C3a. Therefore, we suggest that heparin and C5a may have a synergistic effect on the aggregation of polymorphonuclear leukocytes. These data might be relevant to situations in which patients are subjected to extracorporeal circulation.
在体外循环过程中，已知一些患者会发生白细胞聚集。这归因于补体激活和趋化因子C5a的释放。由于大多数患者在体外循环期间接受肝素化，我们研究了肝素对体外补体诱导的多形核白细胞聚集（PMNAGG）的影响。我们的结果表明，酵母聚糖激活血清（ZAS）的亚聚集浓度会增强肝素诱导的PMNAGG，而亚聚集量的肝素会增加ZAS诱导的聚集。酵母聚糖激活前血清的热灭活消除了ZAS的聚集活性。此外，ZAS和肝素联合诱导的PMNAGG被抗C5a特异性抗体部分抑制，但抗C3a抗体则无此作用。因此，我们认为肝素和C5a可能对多形核白细胞的聚集具有协同作用。这些数据可能与患者接受体外循环的情况相关。
