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从墨西哥北部两种鹿类动物体上采集的白纹革蜱的分子鉴定和形态变异。

Molecular identification and morphological variations of Dermacentor albipictus collected from two deer species in northern Mexico.

机构信息

Departamento en Ciencias Veterinarias, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Cd. Juárez, Chihuahua, Mexico.

Facultad de Ciencias Veterinarias, Instituto de Ecología y Evolución, Universidad Austral de Chile, Valdivia, Chile.

出版信息

Exp Appl Acarol. 2021 Jun;84(2):473-484. doi: 10.1007/s10493-021-00613-7. Epub 2021 Jun 5.

DOI:10.1007/s10493-021-00613-7
PMID:34089463
Abstract

In total, 57 ticks were collected from six white-tailed deer (Odocoileus virginianus) and three mule deer (O. hemionus) in northern Mexico during the 2017, 2018 and 2019 hunting seasons. Morphological features of adult male and female ticks were observed and photographed using a stereo-microscope and scanning electron micrography. The ticks were identified as Dermacentor albipictus based on taxonomic keys. Molecular analysis using DNA amplification of the 16S rDNA and cytochrome oxidase 1 (COI) genes was employed to resolve the phylogenetic relationships from 18 strains of Dermacentor species. Bayesian phylogenetic analysis was performed in order to obtain a phylogenetic tree based on the concatenated sequence in the D. albipictus clade. The geometric morphometric analysis compared the body shape of ticks collected from specimens of two deer species by analyzing nine dorsal and ventral landmarks from both males and females. The results suggest that body shape variation in dorsal structures might be related to the host.

摘要

在 2017 年、2018 年和 2019 年狩猎季节期间,在墨西哥北部共从 6 只白尾鹿(Odocoileus virginianus)和 3 只骡鹿(O. hemionus)身上采集了 57 只蜱虫。使用立体显微镜和扫描电子显微镜观察并拍摄了成年雄性和雌性蜱虫的形态特征。根据分类学关键特征,这些蜱虫被鉴定为白纹革蜱。使用 16S rDNA 和细胞色素氧化酶 1(COI)基因的 DNA 扩增进行分子分析,以解决来自 18 株革蜱物种的系统发育关系。进行贝叶斯系统发育分析,以根据白纹革蜱分支的串联序列获得系统发育树。通过分析来自雄性和雌性的九个背部和腹部标志点,对来自两种鹿种标本的蜱虫的身体形状进行了几何形态测量分析。结果表明,背部结构的体型变化可能与宿主有关。

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