Jin Jian, Tang Tao, Zhou Hao, Hong Xu-Dong, Fan Hao, Zhang Xu-Dong, Chen Zheng-Li, Ma Bing, Zhu Shi-Hui, Wang Guang-Yi, Xia Zhao-Fan
Department of Burn & Plastic Surgery, 903rd Hospital of PLA (The Original 117th Hospital of People's Liberation Army), Hangzhou, Zhejiang, China.
Department of Burn Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China.
J Burn Care Res. 2022 Mar 23;43(2):445-452. doi: 10.1093/jbcr/irab100.
Both silicone gel and quercetin are effective in scar treatment but have different action mechanisms. Quercetin is mainly applied in the gel form and can lead to poor adhesion of silicone gel sheet; therefore, they cannot be combined in clinical use. In this study, a silicone gel sheet that releases quercetin in a sustained manner for 48 hours was successfully developed. Four round scars (Ø: 1 cm) were made in the ears of New Zealand albino rabbits (n = 10). After scar healing, the rabbits were divided into four groups: blank control group with no treatment, silicone gel sheet group with dressing change every 2 days, quercetin group with dressing change three times daily, and combination treatment group with dressing change every 2 days. Scar assessment was performed 3 months later. Transepidermal water loss showed no difference between the combination treatment group and the silicone gel sheet group, but was lower than that in the quercetin group and the blank control group. Immunohistochemistry of CD 31 and proliferating cell nuclear antigen showed the following results: combination treatment group < silicone gel sheet group = quercetin group < blank control group. Polymerase chain reaction results showed that the expression of type-I and type-III collagen in the combination treatment group and the quercetin group was significantly lower than that in the other two groups. Thus, quercetin-modified silicone gel sheet combines the advantages of the two treatments and is more effective at inhibiting cell proliferation in scar tissue than either of the two treatments alone.
硅胶凝胶和槲皮素在瘢痕治疗中均有效，但作用机制不同。槲皮素主要以凝胶形式应用，会导致硅胶凝胶片的黏附性差；因此，它们在临床应用中不能联合使用。在本研究中，成功研制出一种能持续48小时释放槲皮素的硅胶凝胶片。在新西兰白化兔（n = 10）的耳部制作4个圆形瘢痕（直径：1 cm）。瘢痕愈合后，将兔子分为四组：未治疗的空白对照组、每2天换药一次的硅胶凝胶片组、每日换药3次的槲皮素组和每2天换药一次的联合治疗组。3个月后进行瘢痕评估。经表皮水分流失显示联合治疗组与硅胶凝胶片组之间无差异，但低于槲皮素组和空白对照组。CD 31和增殖细胞核抗原的免疫组织化学结果如下：联合治疗组<硅胶凝胶片组 = 槲皮素组<空白对照组。聚合酶链反应结果显示，联合治疗组和槲皮素组中I型和III型胶原蛋白的表达明显低于其他两组。因此，槲皮素修饰的硅胶凝胶片结合了两种治疗方法的优点，在抑制瘢痕组织中的细胞增殖方面比单独使用两种治疗方法中的任何一种更有效。
