Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, China.
Chengdu Academy of Environmental Sciences, Chengdu, China.
Luminescence. 2021 Sep;36(6):1553-1560. doi: 10.1002/bio.4100. Epub 2021 Jun 15.
Tetracycline (TC) is a broad-spectrum antibiotic used to treat bacterial infections. In this study, a ratiometric fluorescence (FL) probe was developed to detect TC in water samples using waste printing paper extract as a FL indicator. For this ratiometric probe, the emission of printing paper extract at 436 nm gradually decreased and the emission of a mixed solution at 538 nm significantly increased with the sequential addition of TC upon excitation at 390 nm, coupled with a marked FL colour change from bright blue to faint yellow. Therefore, a ratiometric F /F FL probe was created for TC detection by simply mixing the printing paper extraction and TC. Under the optimized conditions, a linear range from 1 to 100 μM and a detection limit of 0.48 μM (S/N = 3) for TC were obtained. Importantly, the FL probe can be easily prepared with rapid response, high sensitivity, and good selectivity. The application of waste printing paper extract for detection of TC in environmental water samples was demonstrated.
四环素（TC）是一种广谱抗生素，用于治疗细菌感染。在这项研究中，我们开发了一种比率荧光（FL）探针，使用废打印纸提取物作为 FL 指示剂来检测水样中的 TC。对于这种比率探针，在 390nm 激发下，随着 TC 的顺序加入，打印纸提取物在 436nm 处的发射逐渐减少，而混合溶液在 538nm 处的发射显著增加，同时伴有明显的 FL 颜色从亮蓝色变为淡黄色的变化。因此，通过简单地混合打印纸提取物和 TC，创建了用于 TC 检测的比率 F/F FL 探针。在优化条件下，TC 的线性范围为 1 至 100μM，检测限为 0.48μM（S/N=3）。重要的是，FL 探针可以快速响应、高灵敏度和良好选择性地轻松制备。该研究还展示了废打印纸提取物在环境水样中检测 TC 的应用。
