College of Chemistry and Chemical Engineering, Henan Key Laboratory of Function-Oriented Porous Materials, Luoyang Normal University, Luoyang, 471934, China; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471022, China.
College of Chemistry and Chemical Engineering, Henan Key Laboratory of Function-Oriented Porous Materials, Luoyang Normal University, Luoyang, 471934, China.
Talanta. 2023 Dec 1;265:124874. doi: 10.1016/j.talanta.2023.124874. Epub 2023 Jun 22.
Rapid, sensitive and portable analytical methods for on-site detection of tetracycline (TC) in food samples is of critical importance for food safety and public health. In this study, a dual-emission ratio fluorescent probe (Gd@Eu) was prepared and utilized for the detection of tetracycline (TC) by observing the fluorescence color change from blue to red. The detection process exhibits a wide linear range (0-52.0 μM), good selectivity and low detection limit (14 nM). A paper-based probe and a colorimetric card was constructed for the visual detection of TC. Furthermore, a novel and portable detection platform combining smartphone and test strip was exploited for the quantitative and on-site detection of TC in real pork sample. The developed method was validated through intra- (n = 5) and inter-day (n = 2) measurements, as well as comparison with a traditional HPLC method. These statistical result validate the reliability and accuracy of the developed method. This intelligent detection platform represents a promising approach for the rapid, sensitive and visual detection of TC in food samples.
快速、灵敏、便携的现场分析方法对于食品中四环素（TC）的检测具有重要意义，这对食品安全和公众健康至关重要。本研究制备了双发射比率荧光探针（Gd@Eu），通过观察荧光颜色从蓝色到红色的变化，实现了对四环素（TC）的检测。检测过程呈现出较宽的线性范围（0-52.0 μM）、良好的选择性和较低的检测限（14 nM）。构建了基于纸的探针和比色卡，用于 TC 的可视化检测。此外，还开发了一种结合智能手机和测试条的新型便携式检测平台，用于实际猪肉样品中 TC 的定量和现场检测。通过日内（n=5）和日间（n=2）测量以及与传统 HPLC 方法的比较，对所开发的方法进行了验证。这些统计结果验证了所开发方法的可靠性和准确性。这种智能检测平台为食品样品中 TC 的快速、灵敏和可视化检测提供了一种有前途的方法。
