State Key Laboratory of Ecohydraulic Engineering in Shaanxi, Xi'an University of Technology, Xi'an 710048, China.
Environ Sci Pollut Res Int. 2013 Apr;20(4):2484-91. doi: 10.1007/s11356-012-1168-6. Epub 2012 Sep 8.
The objective of this study was to investigate the impacts of exogenous nitrogen on a microbial community inoculated with the atrazine-degrading Arthrobacter sp. in soil amended with a high concentration of atrazine. Inoculated and uninoculated microcosms for biodegradation tests were constructed. Atrazine degradation capacity of the strain DAT1 and the strain's atrazine-metabolic potential and survival were assessed. The relative abundance of the strain DAT1 and the bacterial community structure in soils were characterized using quantitative PCR in combination with terminal restriction fragment length polymorphism. Atrazine degradation by the strain DAT1 and the strain's atrazine-metabolic potential and survival were not affected by addition of a medium level of nitrate, but these processes were inhibited by addition of a high level of nitrate. Microbial community structure changed in both inoculated and uninoculated microcosms, dependent on the level of added nitrate. Bioaugmentation with the strain DAT1 could be a very efficient biotechnology for bioremediation of soils with high concentrations of atrazine.
本研究旨在探讨在添加高浓度莠去津的土壤中接种莠去津降解菌节杆菌（Arthrobacter sp.）时，外源氮对微生物群落的影响。构建了用于生物降解试验的接种和未接种微宇宙。评估了菌株 DAT1 的莠去津降解能力以及菌株的莠去津代谢潜能和生存能力。利用定量 PCR 结合末端限制性片段长度多态性技术，对土壤中菌株 DAT1 的相对丰度和细菌群落结构进行了表征。添加中等水平的硝酸盐不会影响菌株 DAT1 的莠去津降解能力、菌株的莠去津代谢潜能和生存能力，但会抑制高浓度硝酸盐的添加。接种和未接种微宇宙中的微生物群落结构都随着添加硝酸盐水平的变化而变化。利用菌株 DAT1 进行生物强化可能是一种非常有效的生物技术，可用于修复高浓度莠去津的土壤。
