Nilbert M, Mandahl N, Heim S, Rydholm A, Willén H, Akerman M, Mitelman F
Department of Clinical Genetics, University Hospital, Lund, Sweden.
Cancer Genet Cytogenet. 1988 Sep;34(2):209-18. doi: 10.1016/0165-4608(88)90262-2.
Short-term cultures from seven soft tissue leiomyosarcomas were investigated cytogenetically. Sufficient mitoses for chromosome analysis were obtained in six cases, four of which had only normal karyotypes. In one tumor, an intramuscular leiomyosarcoma of the lower arm, a variety of nonclonal structural and numerical aberrations were found in two thirds of the metaphases. Another tumor, a subcutaneous leiomyosarcoma of the knee, had clonal abnormalities resulting in the karyotype 46,X,der(X)t(X;4)(:Xq26----cen----Xp22::4q23----4qter) , del(4)(q23)/47,X,der(X)t(X;4),del(4)(q23), + 20. Flow cytofluorometric measurements of the DNA content in the six leiomyosarcomas successfully karyotyped revealed diploid values in five tumors. The leiomyosarcoma displaying numerous nonclonal changes had two cytofluorometric peaks, 1.01 and 1.39, indicating that the metaphases available for cytogenetic study cannot have been fully representative of the tumor stemline.
对7例软组织平滑肌肉瘤进行了短期培养，并进行了细胞遗传学研究。6例获得了足够用于染色体分析的有丝分裂细胞，其中4例核型正常。在1例肿瘤（一例前臂肌内平滑肌肉瘤）中，三分之二的中期细胞发现了多种非克隆性结构和数量异常。另一例肿瘤（一例膝部皮下平滑肌肉瘤）有克隆性异常，核型为46,X,der(X)t(X;4)(:Xq26----cen----Xp22::4q23----4qter) ，del(4)(q23)/47,X,der(X)t(X;4),del(4)(q23), + 20。对成功进行核型分析的6例平滑肌肉瘤进行的DNA含量流式细胞荧光测定显示，5例肿瘤为二倍体值。显示大量非克隆性变化的平滑肌肉瘤有两个细胞荧光测定峰，分别为1.01和1.39，这表明可用于细胞遗传学研究的中期细胞不可能完全代表肿瘤主干系。
