Gong Hongli, Wang Boyan, Shi Yi, Shi Yong, Xiao Xiyan, Cao Pengyu, Tao Lei, Wang Yuezhu, Zhou Liang
Shanghai Key Clinical Disciplines of Otorhinolaryngology, Department of Otorhinolaryngology, Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai, 200031, P. R. China.
Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
J Microbiol. 2017 Aug;55(8):648-654. doi: 10.1007/s12275-017-6636-8. Epub 2017 Jul 28.
The pharynx is an important site of microbiota colonization, but the bacterial populations at this site have been relatively unexplored by culture-independent approaches. The aim of this study was to characterize the microbiota structure of the pharynx. Pyrosequencing of 16S rRNA gene libraries was used to characterize the pharyngeal microbiota using swab samples from 68 subjects with laryngeal cancer and 28 subjects with vocal cord polyps. Overall, the major phylum was Firmicutes, with Streptococcus as the predominant genus in the pharyngeal communities. Nine core operational taxonomic units detected from Streptococcus, Fusobacterium, Prevotella, Granulicatella, and Veillonella accounted for 21.3% of the total sequences detected. However, there was no difference in bacterial communities in the pharynx from patients with laryngeal cancer and vocal cord polyps. The relative abundance of Firmicutes was inversely correlated with Fusobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes. The correlation was evident at the genus level, and the relative abundance of Streptococcus was inversely associated with Fusobacterium, Leptotrichia, Neisseria, Actinomyces, and Prevotella. This study presented a profile for the overall structure of the microbiota in pharyngeal swab samples. Inverse correlations were found between Streptococcus and other bacterial communities, suggesting that potential antagonism may exist among pharyngeal microbiota.
咽是微生物群定植的重要部位，但通过非培养方法对该部位细菌群落的研究相对较少。本研究旨在描述咽微生物群的结构。利用16S rRNA基因文库焦磷酸测序技术，对68例喉癌患者和28例声带息肉患者的咽拭子样本进行分析，以描述咽微生物群特征。总体而言，主要菌门为厚壁菌门，链球菌是咽群落中的优势菌属。从链球菌、梭杆菌、普雷沃菌、颗粒链菌和韦荣球菌中检测到的9个核心操作分类单元占检测到的总序列的21.3%。然而，喉癌患者和声带息肉患者咽内的细菌群落并无差异。厚壁菌门的相对丰度与梭杆菌门、变形菌门、放线菌门和拟杆菌门呈负相关。这种相关性在属水平上很明显，链球菌的相对丰度与梭杆菌、纤毛菌、奈瑟菌、放线菌和普雷沃菌呈负相关。本研究展示了咽拭子样本中微生物群的整体结构概况。发现链球菌与其他细菌群落之间存在负相关，提示咽微生物群之间可能存在潜在拮抗作用。
