de Oliveira Maykon Tavares, Sulleiro Elena, da Silva Maria Cláudia, Silgado Aroa, de Lana Marta, da Silva João Santana, Molina Israel, Marin-Neto J Antônio
Department of Infectious Diseases, Vall d'Hebron University Hospital, Universitat Autònoma de Barcelona, PROSICS Barcelona, Barcelona, Spain.
Cardiology Division, Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Front Cardiovasc Med. 2021 May 20;8:665624. doi: 10.3389/fcvm.2021.665624. eCollection 2021.
has a high rate of biological and genetic variability, and its population structure is divided into seven distinct genetic groups (TcI-TcVI and Tcbat). Due to immigration, Chagas disease (ChD), caused by , has become a serious global health problem including in Europe. Therefore, the aim of this study was to evaluate the existence of genetic variability within discrete typing unit (DTU) TcV of in Bolivian patients with chronic ChD residing in Barcelona, Spain. The DNA was extracted from the peripheral blood of 27 patients infected with DTU TcV and the fragments of the genetic material were amplificated through the low stringency single primer-polymerase chain reaction (LSSP-PCR). The data generated after amplification were submitted to bioinformatics analysis. Of the 27 patients evaluated in the study, 8/27 (29.6%) were male and 19/27 (70.4%) female, 17/27 (62.9%) were previously classified with the indeterminate clinical form of Chagas disease and 10/27 (37.1%) with Chagas cardiomyopathy. The LSSP-PCR detected 432 band fragments from 80 to 1,500 bp. The unweighted pair-group method analysis and principal coordinated analysis data demonstrated the existence of three distinct genetic groups with moderate-high rates of intraspecific genetic variability/diversity that had shared parasite's alleles in patients with the indeterminate and cardiomyopathy forms of ChD. This study demonstrated the existence of a moderate to high rate of intra-DTU TcV variability in . Certain alleles of the parasite were associated with the absence of clinical manifestations in patients harboring the indeterminate form of ChD. These results support the need to search for increasingly specific targets in the genome of to be correlated with its main biological properties and clinical features in patients with chronic ChD.
具有很高的生物学和遗传变异性，其种群结构分为七个不同的遗传组（TcI - TcVI和Tcbat）。由于移民，由其引起的恰加斯病（ChD）已成为包括欧洲在内的严重全球健康问题。因此，本研究的目的是评估西班牙巴塞罗那患有慢性ChD的玻利维亚患者中，克氏锥虫离散分型单元（DTU）TcV内的遗传变异性。从27例感染克氏锥虫DTU TcV的患者外周血中提取DNA，并通过低严谨度单引物聚合酶链反应（LSSP - PCR）扩增遗传物质片段。扩增后生成的数据进行生物信息学分析。在该研究评估的27例患者中，8/27（29.6%）为男性，19/27（70.4%）为女性，17/27（62.9%）先前被归类为恰加斯病的不确定临床形式，10/27（37.1%）患有恰加斯心肌病。LSSP - PCR检测到80至1500 bp的432条条带片段。非加权配对组法分析和主坐标分析数据表明，存在三个不同的遗传组，种内遗传变异性/多样性中等偏高，在患有不确定和心肌病形式ChD的患者中存在共享的寄生虫等位基因。本研究证明了克氏锥虫DTU TcV内存在中等至高变异性。寄生虫的某些等位基因与患有不确定形式ChD的患者无临床表现相关。这些结果支持需要在克氏锥虫基因组中寻找越来越特异的靶点，以与其在慢性ChD患者中的主要生物学特性和临床特征相关联。
