Yoshida Yuki, Horikawa Daiki D, Sakashita Tetsuya, Yokota Yuichiro, Kobayashi Yasuhiko, Tomita Masaru, Arakawa Kazuharu
Keio University, Institute for Advanced Biosciences, Tsuruoka, Yamagata 997-0035, Japan.
Keio University, Graduate School of Media and Governance, Systems Biology Program, Fujisawa, Kanagawa 252-0882, Japan.
Data Brief. 2021 May 9;36:107111. doi: 10.1016/j.dib.2021.107111. eCollection 2021 Jun.
Tardigrades are microscopic animals of which terrestrial species are capable of tolerating extreme environments by entering a desiccated ametabolic state known as anhydrobiosis. Intriguingly, they survive high dosage gamma rays (>4,000 Gy), possibly through a mechanism known as cross-tolerance. We hypothesized that anhydrobiosis genes are also regulated during cross-tolerance, thus we submitted to 500 Gy Co gamma-ray and conducted time-course low-input RNA-Seq. The gene expression was quantified with RSEM and differential expression was determined with DEseq2. Differentially expressed genes were submitted to gene ontology enrichment analysis with GOStat. The transcriptome dynamically shifted nine hours post-exposure.
水熊虫是一种微观动物，其中陆生种类能够通过进入一种称为脱水隐生的干燥无代谢状态来耐受极端环境。有趣的是，它们能在高剂量伽马射线（>4000戈瑞）下存活，可能是通过一种称为交叉耐受的机制。我们推测脱水隐生基因在交叉耐受过程中也受到调控，因此我们将其暴露于500戈瑞的钴伽马射线下，并进行了时间进程低输入RNA测序。用RSEM对基因表达进行定量，用DEseq2确定差异表达。将差异表达基因提交给GOStat进行基因本体富集分析。暴露后9小时转录组发生动态变化。
