凝胶（Hydragel 30）电泳和毛细管（Capillarys III Tera）电泳用于人血清蛋白表征的比较

A Comparison of gel (Hydragel 30) and capillary (Capillarys III Tera) electrophoresis for the characterization of human serum proteins.

作者信息

Howard Brittney M, Kuh Annie, Rezavi LuAnn, Caturegli Patrizio

机构信息

Division of Immunology, Department of Pathology, The Johns Hopkins School of Medicine, Ross Building-Room 656, 720 Rutland Avenue, Baltimore, MD, 21205, USA.

出版信息

Pract Lab Med. 2021 Apr 29;25:e00233. doi: 10.1016/j.plabm.2021.e00233. eCollection 2021 May.

DOI:10.1016/j.plabm.2021.e00233

PMID:34095418

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8145771/

Abstract

OBJECTIVES

To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins.

DESIGN AND METHODS

304 sera tested by gel electrophoresis during 8 routine laboratory days were concurrently tested by capillary electrophoresis. Gels were read by an experienced medical technologist while capillary profiles by a Sebia representative and the same technologist. Most sera (214 of 304, 70%) were also analyzed by immunofixation electrophoresis, used here as the gold standard to calculate sensitivity and specificity of the gel and capillary systems.

RESULTS

Gel and capillary estimated the concentration of albumin, gamma region, and M-spikes nearly perfectly, and that of beta, alpha-2, and alpha-1 regions with excellent correlation. The two systems classified concordantly 268 of 304 sera (88% agreement) as having no, one, or two M-spikes, but differed in the remaining 36 sera (12%). Gel electrophoresis correctly identified M-spikes in 82 of 112 sera that were shown to have monoclonal band(s) by immunofixation (73% sensitivity), and correctly did not reveal M-spikes in 97 of the 102 sera that had no immunofixation bands (95% specificity). Capillary achieved slightly higher sensitivity (85 of 112, 76%) and slightly lower specificity (94 of 102, 92%), but the two areas under the ROC curves were nearly identical at 0.84.

CONCLUSIONS

Gel and capillary electrophoresis systems perform similarly to estimate the concentration of serum protein fractions and detect M-spikes.

摘要

目的

比较凝胶电泳（Sebia公司的Hydrasys 2）和毛细管电泳（Sebia公司的Capillarys III Tera）对人血清蛋白的特征分析。

设计与方法

在8个常规实验室工作日中，对304份经凝胶电泳检测的血清同时进行毛细管电泳检测。凝胶电泳结果由经验丰富的医学技术人员读取，毛细管电泳图谱由Sebia公司代表和同一名技术人员读取。大多数血清（304份中的214份，占70%）还通过免疫固定电泳进行分析，在此用作计算凝胶电泳和毛细管电泳系统敏感性和特异性的金标准。

结果

凝胶电泳和毛细管电泳对白蛋白、γ区和M峰浓度的估计几乎完全一致，对β区、α-2区和α-1区浓度的估计具有极好的相关性。两种系统对304份血清中的268份（一致性为88%）分类一致，即无M峰、有一个M峰或有两个M峰，但在其余36份血清（12%）中存在差异。在112份经免疫固定电泳显示有单克隆条带的血清中，凝胶电泳正确识别出82份有M峰（敏感性为73%）；在102份无免疫固定条带的血清中，凝胶电泳正确显示97份无M峰（特异性为95%）。毛细管电泳的敏感性略高（112份中的85份，占76%），特异性略低（102份中的94份，占92%），但两条ROC曲线下的面积几乎相同，均为0.84。