Bossuyt Xavier, Lissoir Bénédicte, Mariën Godelieve, Maisin Diane, Vunckx Jozef, Blanckaert Norbert, Wallemacq Pierre
Laboratory Medicine, Immunology, University Hospital Leuven, Leuven, Belgium.
Clin Chem Lab Med. 2003 May;41(5):704-10. doi: 10.1515/CCLM.2003.107.
Capillary zone electrophoresis (CZE) of serum proteins is increasingly gaining impact in clinical laboratories. In this report, we evaluate automated capillary zone electrophoresis by Capillarys (Sebia, France). Within-run and between-run imprecision for the five electrophoretic fractions was <2% and <6%, respectively. Data obtained with Capillarys correlated with results obtained with agarose gel electrophoresis and Paragon CZE 2000 (Beckman Coulter, USA). Analysis of serum obtained from patients with inflammation, nephrotic syndrome, bisalbuminemia, and alpha1-antitrypsin deficiency revealed that Capillarys was able to detect these abnormalities. Two hundred thirty eight samples were analyzed by agarose gel electrophoresis, Capillarys, capillary electrophoresis using Paragon CZE 2000 system, and immunofixation. Sample selection was based on the presence of a disturbed morphology (e.g., spike) of the protein profile or hypogammaglobulinemia on agarose gel electrophoresis and/or Capillarys. Immunofixation revealed the presence of a monoclonal protein, oligoclonal bands, polyclonal pattern, and a normal profile in, respectively, 89, 66, 19, and 64 samples. With Capillarys, Paragon, and agarose gel electrophoresis, a spike and/or disturbed morphology of the profile was found in 222, 182, and 180 samples, respectively. In these samples, immunofixation was negative in 73 (33%), 46 (25%), and 39 (22%) samples, respectively. These data indicate that Capillarys has a lower specificity than agarose gel electrophoresis and Paragon 2000. Of the 89 samples with a monoclonal protein, Capillarys, Paragon, and agarose gel electrophoresis failed to detect, respectively, three, three, and one monoclonal protein(s). Interferences by radio-opaque agents, complement degradation products, fibrinogen, and triglycerides are described. In conclusion, automated capillary zone electrophoresis with Capillarys provides for reproducible, rapid, and reliable serum electrophoresis.
血清蛋白的毛细管区带电泳（CZE）在临床实验室中的影响力日益增强。在本报告中，我们对法国Sebia公司的Capillarys全自动毛细管区带电泳仪进行了评估。五个电泳组分的批内不精密度和批间不精密度分别<2%和<6%。Capillarys获得的数据与琼脂糖凝胶电泳和美国贝克曼库尔特公司的Paragon CZE 2000获得的结果相关。对炎症、肾病综合征、双白蛋白血症和α1抗胰蛋白酶缺乏症患者的血清分析表明，Capillarys能够检测到这些异常情况。采用琼脂糖凝胶电泳、Capillarys、使用Paragon CZE 2000系统的毛细管电泳和免疫固定法对238份样本进行了分析。样本选择基于琼脂糖凝胶电泳和/或Capillarys上蛋白质图谱形态异常（如尖峰）或低丙种球蛋白血症的存在情况。免疫固定法分别在89、66、19和64份样本中检测到单克隆蛋白、寡克隆带、多克隆模式和正常图谱。使用Capillarys、Paragon和琼脂糖凝胶电泳时，分别在222、182和180份样本中发现了图谱的尖峰和/或形态异常。在这些样本中，免疫固定法分别在73份（33%）、46份（25%）和39份（22%）样本中呈阴性。这些数据表明，Capillarys的特异性低于琼脂糖凝胶电泳和Paragon 2000。在89份含有单克隆蛋白的样本中，Capillarys、Paragon和琼脂糖凝胶电泳分别未能检测到3份、3份和1份单克隆蛋白。文中描述了造影剂、补体降解产物、纤维蛋白原和甘油三酯的干扰情况。总之，使用Capillarys的全自动毛细管区带电泳可提供可重复、快速且可靠的血清电泳。
