Roth T P, Hubbard A K, Gandolfi A J, Brown B R
Department of Anesthesiology, University of Arizona, Tucson 85724.
Clin Exp Immunol. 1988 May;72(2):330-6.
Multiple halothane exposures in rabbits generate modified liver proteins or antigens that appear to incorporate the metabolic intermediate of halothane, trifluoroacetyl halide (TFA), as identified by specific anti-TFA antibody. These halothane-induced antigens are most prevalent throughout the second to fourth days following a single halothane exposure and are in highest concentration after the second and third exposure. In addition, five consecutive halothane exposures at 2-week intervals caused the sustained expression of these halothane-induced antigens throughout the first 4 days following the last exposure. By the seventh day, however, antigen expression began to decline. Although there is great heterogeneity in the molecular weights of the halothane-induced antigens, the predominant proteins appear to be 85k, 58k, 53k, 37k and 24k. These liver proteins could reflect self proteins altered by trifluoroacetylation by halothane metabolites and may be potential immunogens in the initiation of a halothane-induced immune response.
兔多次接触氟烷会产生修饰的肝脏蛋白质或抗原，这些蛋白质或抗原似乎结合了氟烷的代谢中间体三氟乙酰卤（TFA），这是通过特异性抗TFA抗体鉴定出来的。这些氟烷诱导的抗原在单次接触氟烷后的第二天至第四天最为普遍，在第二次和第三次接触后浓度最高。此外，每隔2周连续5次接触氟烷会导致这些氟烷诱导的抗原在最后一次接触后的前4天持续表达。然而，到第7天，抗原表达开始下降。尽管氟烷诱导的抗原分子量存在很大异质性，但主要蛋白质似乎是85k、58k、53k、37k和24k。这些肝脏蛋白质可能反映了被氟烷代谢产物三氟乙酰化改变的自身蛋白质，并且可能是引发氟烷诱导的免疫反应的潜在免疫原。
