Ma Hongshi, Yu Qingqing, Qu Yu, Zhu Yufang, Wu Chengtie
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, No,19(A) Yuquan Road, Beijing, 100049, China.
Bioact Mater. 2021 May 15;6(12):4558-4567. doi: 10.1016/j.bioactmat.2021.04.042. eCollection 2021 Dec.
To prevent postoperative skin tumor recurrence and repair skin wound, a glucose oxidase (GOx)-loaded manganese silicate hollow nanospheres (MS HNSs)-incorporated alginate hydrogel (G/MS-SA) was constructed for starvation-photothermal therapy and skin tissue regeneration. The MS HNSs showed a photothermal conversion efficiency of 38.5%, and endowed composite hydrogels with satisfactory photothermal effect. Taking advantage of the catalytic activity of Mn ions, the composite hydrogels could decompose hydrogen peroxide (HO) into oxygen (O), which can alleviate the problem of tumor hypoxia microenvironment and endow GOx with an ability to consume glucose in the presence of O for tumor starvation. Meanwhile, hyperthermia triggered by near infrared (NIR) irradiation could not only accelerate the reaction rate of HO decomposition by MS HNSs and glucose consumption by GOx, but also ablate tumor cells. The anti-tumor results showed that synergistic effect of starvation-photothermal therapy led to the highest death rate of tumor cells among all groups, and its anti-tumor effect was obviously improved as compared with that of single photothermal treatment or starvation treatment. Interestingly, the introduction of MS HNSs into hydrogels could distinctly promote the epithelialization of the wound beds by releasing Mn ions as compared with the hydrogels without MS HNSs. It is expected that such a multifunctional platform with starvation-photothermal therapy will be promising for treating tumor-caused skin defects in combination of its regeneration bioactivity in the future.
为防止术后皮肤肿瘤复发并修复皮肤伤口，构建了一种负载葡萄糖氧化酶（GOx）的硅酸锰中空纳米球（MS HNSs）复合藻酸盐水凝胶（G/MS-SA），用于饥饿-光热疗法和皮肤组织再生。MS HNSs的光热转换效率为38.5%，赋予复合水凝胶令人满意的光热效应。利用锰离子的催化活性，复合水凝胶可将过氧化氢（HO）分解为氧气（O），这可以缓解肿瘤缺氧微环境问题，并使GOx在有O的情况下具有消耗葡萄糖以实现肿瘤饥饿的能力。同时，近红外（NIR）照射引发的热疗不仅可以加速MS HNSs分解HO和GOx消耗葡萄糖的反应速率，还可以消融肿瘤细胞。抗肿瘤结果表明，饥饿-光热疗法的协同效应导致所有组中肿瘤细胞的死亡率最高，与单一光热治疗或饥饿治疗相比，其抗肿瘤效果明显提高。有趣的是，与不含MS HNSs的水凝胶相比，将MS HNSs引入水凝胶中可通过释放锰离子显著促进伤口床的上皮形成。预计这种具有饥饿-光热疗法的多功能平台在未来结合其再生生物活性治疗肿瘤引起的皮肤缺损方面将具有广阔前景。
