State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy and Food Company Ltd., Shanghai, China.
Department of Pharmacy, Nanjing Medical University Affiliated Wuxi No. 2 People's hospital, Wuxi, China.
J Food Sci. 2021 Jun;86(6):2684-2699. doi: 10.1111/1750-3841.15761. Epub 2021 Jun 6.
Nowadays, consumers have an increasing demand for health products. In this study, an oral liquid was developed using a compound extract consisting of three herbal extracts (Dendrobium nobile Lindl., Lycium barbarum, and Puerariae lobatae Radix) because the compound extract (a combination of all three extracts) was superior to every single extract in promoting the phagocytic capacity of RAW264.7 macrophages and the proliferation ability of GES-1 cells. In this oral liquid, the dosage of the stabilizer and the sweetener was selected using a stability test and sensory quality evaluation. When 0.30% (m/v) xanthan gum and 0.20% (m/v) mogroside were added, the oral liquid had not only a good stability but also the highest sensory score for overall acceptability. The chemical composition analysis showed that the oral liquid had various functional ingredients including polysaccharides, phenols, alkaloids, and so forth. The immune-enhancing efficacy of the oral liquid was evaluated in BALB/c mice by measuring the levels of different immune indicators. The results indicated that the oral liquid obviously enhanced nonspecific and specific immunity. A rat model with ethanol-induced gastric ulcer was used to examine the protective effect of the oral liquid on the gastric mucosa and to explore the related mechanisms. The oral administration of the oral liquid for days significantly prevented the formation of gastric ulcer. This study provided an effective oral liquid that could enhance immunity and protect gastric mucosa.
如今，消费者对保健品的需求日益增长。在这项研究中，我们开发了一种口服液，该口服液使用由三种草药提取物（铁皮石斛、枸杞和葛根）组成的复方提取物，因为复方提取物（三种提取物的组合）在促进 RAW264.7 巨噬细胞的吞噬能力和 GES-1 细胞的增殖能力方面优于单一提取物。在这种口服液中，通过稳定性试验和感官质量评估选择了稳定剂和甜味剂的用量。当添加 0.30%（m/v）黄原胶和 0.20%（m/v）罗汉果甜苷时，口服液不仅具有良好的稳定性，而且整体可接受性的感官评分最高。化学成分分析表明，口服液含有多种具有免疫调节作用的功能性成分，包括多糖、酚类、生物碱等。通过测量不同免疫指标，在 BALB/c 小鼠中评估了口服液的免疫增强功效。结果表明，口服液明显增强了非特异性和特异性免疫。使用乙醇诱导的大鼠胃溃疡模型，考察口服液对胃黏膜的保护作用及其相关机制。口服液连续给药数天可显著预防胃溃疡的形成。本研究提供了一种有效的口服液，可增强免疫力并保护胃黏膜。
