College of Veterinary Medicine, Northwest A&F University, Yangling, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Anim Biotechnol. 2023 Feb;34(1):8-14. doi: 10.1080/10495398.2021.1929272. Epub 2021 Jun 7.
The ovary can generate oocytes and secrete female hormones and thus is of great significance to animal fertility. In turn, the functioning of this organ has an effect on the profit margins of the livestock breeding industry. As the development-regulating gene and target gene of miR-202, might play an important role in ovarian growth. Therefore, we hypothesized that is related to ovarian traits owing to the regulation of gonad-specific miR-202. To further investigate the connection between bovine and ovarian development, we analyzed data from 408 samples. After genotyping and analyzing three selected loci, we found that two out of the three loci (L1 and L5) were polymorphic, of which the minimum allelic frequencies were 0.417 (L1) and 0.094 (L5). Moreover, one novel indel L1 of was associated with ovarian length ( < 0.05). More specifically, individuals with II and ID genotypes have longer ovaries than those with the DD genotype. Our work shows that can be selected as a testing marker gene for animal fertility. Our findings contribute to improving the prospects of the cattle industry and the wider use of genetic techniques in breeding.
卵巢可以产生卵子并分泌女性激素，因此对动物的生育能力具有重要意义。反过来，该器官的功能对畜牧业的利润率也有影响。作为 miR-202 的发育调节基因和靶基因，可能在卵巢生长中发挥重要作用。因此，我们假设由于性腺特异性 miR-202 的调节，与卵巢特征有关。为了进一步研究牛与卵巢发育之间的联系，我们分析了 408 个样本的数据。在对三个选定的基因座进行基因分型和分析后，我们发现其中两个基因座（L1 和 L5）是多态的，最小等位基因频率分别为 0.417（L1）和 0.094（L5）。此外，还发现了一个与卵巢长度相关的 novel indel L1（<0.05）。具体来说，II 和 ID 基因型个体的卵巢比 DD 基因型个体的卵巢更长。我们的工作表明，可以选择作为动物生育能力的检测标记基因。我们的研究结果有助于提高牛业的前景和更广泛地利用遗传技术进行繁殖。
