Deakin University, IMPACT - the Institute for Mental and Physical Health and Clinical Translation, Barwon Health, Geelong, VIC, 3220, Australia; Murdoch Children's Research Institute, Parkville, Victoria, 3052, Australia.
Deakin University, IMPACT - the Institute for Mental and Physical Health and Clinical Translation, Barwon Health, Geelong, VIC, 3220, Australia; Murdoch Children's Research Institute, Parkville, Victoria, 3052, Australia; Black Dog Institute, NSW, Australia.
EBioMedicine. 2021 Jun;68:103400. doi: 10.1016/j.ebiom.2021.103400. Epub 2021 Jun 5.
Murine studies demonstrate that maternal prenatal gut microbiota influences brain development and behaviour of offspring. No human study has related maternal gut microbiota to behavioural outcomes during early life. This study aimed to evaluate relationships between the prenatal faecal microbiota, prenatal diet and childhood behaviour.
A sub-cohort of 213 mothers and 215 children were selected from a longitudinal pre-birth cohort. Maternal prenatal exposure measures collected during the third trimester included the faecal microbiota (generated using 16S rRNA amplicon sequencing), and dietary intake. The behavioural outcome used the Childhood Behaviour Checklist at age two. Models were adjusted for prenatal diet, smoking, perceived stress, maternal age and sample batch.
We found evidence that the alpha diversity of the maternal faecal microbiota during the third trimester of pregnancy predicts child internalising behaviour at two years of age (-2·74, (-4·71, -0·78), p = 0·01 (Wald test), R=0·07). Taxa from butyrate-producing families, Lachnospiraceae and Ruminococcaceae, were more abundant in mothers of children with normative behaviour. A healthy prenatal diet indirectly related to decreased child internalising behaviours via higher alpha diversity of maternal faecal microbiota.
These findings support animal studies showing that the composition of maternal prenatal gut microbiota is related to offspring brain development and behaviour. Our findings highlight the need to evaluate potential impacts of the prenatal gut microbiota on early life brain development.
This study was funded by the National Health and Medical Research Council of Australia (1082307, 1147980), Murdoch Children's Research Institute, Barwon Health and Deakin University.
动物研究表明，母体产前肠道微生物群会影响后代的大脑发育和行为。 尚无人类研究将母体肠道微生物群与生命早期的行为结果联系起来。 本研究旨在评估产前粪便微生物群、产前饮食与儿童行为之间的关系。
从一项纵向产前队列中选择了 213 名母亲和 215 名儿童的子队列。 孕晚期收集的母体产前暴露措施包括粪便微生物群（使用 16S rRNA 扩增子测序生成）和饮食摄入。 使用儿童行为检查表评估 2 岁时的行为结果。 模型调整了产前饮食、吸烟、感知压力、母亲年龄和样本批次。
我们有证据表明，妊娠晚期母体粪便微生物群的 alpha 多样性可预测儿童 2 岁时的内化行为（-2.74，（-4.71，-0.78），p=0.01（Wald 检验），R=0.07）。 丁酸产生菌科、lachnospiraceae 和 ruminococcaceae 的分类群在行为正常儿童的母亲中更为丰富。 健康的产前饮食通过增加母体粪便微生物群的 alpha 多样性，间接地与降低儿童内化行为有关。
这些发现支持动物研究表明，母体产前肠道微生物群的组成与后代大脑发育和行为有关。 我们的研究结果强调了需要评估产前肠道微生物群对生命早期大脑发育的潜在影响。
本研究由澳大利亚国家健康与医学研究理事会（1082307、1147980）、默多克儿童研究所、巴旺卫生区和迪肯大学资助。
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