Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
ACS Appl Mater Interfaces. 2021 Jun 23;13(24):27895-27903. doi: 10.1021/acsami.1c05705. Epub 2021 Jun 8.
Phototherapy holds great promise in the treatment of bacterial infections, especially the multidrug resistant bacterial infections. However, most therapeutic agents are based on the integration of individual photothermal agents and photosensitizers, always in the activated state, and generally lack bacterial specificity, resulting in uncertain pharmacokinetics and serious nonspecific damage to normal tissues. Herein, we report a pH-responsive nanoplatform with synergistic chemo-phototherapy function for smart fluorescence imaging-guided precision sterilization. pH reversible activated symmetric cyanine was designed and prepared as a bacterial-specific imaging unit and PTT/PDT-in-one agent. Meanwhile, a guanidinium-based covalent organic framework (COF) was employed as a nanocarrier and chemotherapy agent to build the intelligent nanoplatform via electrostatic self-assembly. The self-assembly of the PTT/PDT-in-one agent and the COF greatly improves the stability and blood circulation of the PTT/PDT-in-one agent and provides charge-reversed intelligent targeting ability. The developed smart nanoplatform not only enables bacterial-targeted imaging but also possesses chemo/PTT/PDT synergetic high-efficiency bactericidal effects with little side effects, showing great potential in practical applications.
光疗在治疗细菌感染方面具有很大的应用前景，特别是在治疗多重耐药性细菌感染方面。然而，大多数治疗药物都基于单个光热剂和光敏剂的整合，它们始终处于激活状态，而且通常缺乏细菌特异性，导致不确定的药代动力学和对正常组织的严重非特异性损伤。在这里，我们报告了一种具有协同化学-光疗功能的 pH 响应纳米平台，用于智能荧光成像引导的精确杀菌。设计并制备了 pH 可逆激活对称菁染料作为细菌特异性成像单元和 PTT/PDT 一体化试剂。同时，胍基共价有机框架（COF）被用作纳米载体，并通过静电自组装构建智能纳米平台。PTT/PDT 一体化试剂和 COF 的自组装极大地提高了 PTT/PDT 一体化试剂的稳定性和血液循环，并提供了电荷反转的智能靶向能力。所开发的智能纳米平台不仅能够实现细菌靶向成像，而且具有化学/PTT/PDT 协同高效杀菌作用，副作用小，在实际应用中具有很大的潜力。
