Department of Rehabilitation Medicine, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510000, China; Department of Rehabilitation Medicine, Shunde Hospital of Southern Medical University, Foshan 528000, China.
Department of Rehabilitation Medicine, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510000, China.
Int Immunopharmacol. 2021 Aug;97:107824. doi: 10.1016/j.intimp.2021.107824. Epub 2021 Jun 5.
Osteoarthritis is the most common disabling joint disease throughout the world, and the effect of therapy on its course is still unsatisfactory in clinical practice. Recent studies have shown that mesenchymal stem cell (MSC)-derived exosomes can promote cartilage repair and regeneration in osteoarthritis, indicating that these exosomes could be a novel and promising strategy for treating osteoarthritis. This study investigated whether low-intensity pulsed ultrasound (LIPUS) enhances the effects of bone marrow MSC (BMSC)-derived exosomes on cartilage regeneration in osteoarthritis and examined the underlying mechanism. Our results revealed that BMSC-derived exosomes display the typical morphological features of exosomes. LIPUS-mediated BMSC-derived exosomes promoted cartilage regeneration, increased chondrocyte proliferation and extracellular matrix synthesis, suppressed inflammation, and inhibited the interleukin (IL)-1β-induced activation of the nuclear factor kappa B (NF-κB) pathway. In brief, LIPUS enhances the promoting effects of BMSC-derived exosomes on osteoarthritic cartilage regeneration, mainly by strengthening the inhibition of inflammation and further enhancing chondrocyte proliferation and cartilage matrix synthesis. The underlying mechanism could be related to the inhibition of the IL-1β-induced activation of the NF-κB pathway.
骨关节炎是全世界最常见的致残性关节疾病，但其在临床实践中的治疗效果仍不尽人意。最近的研究表明，间充质干细胞（MSC）衍生的外泌体可以促进骨关节炎中的软骨修复和再生，这表明这些外泌体可能是治疗骨关节炎的一种新颖而有前途的策略。本研究探讨了低强度脉冲超声（LIPUS）是否增强骨髓间充质干细胞（BMSC）衍生的外泌体对骨关节炎软骨再生的作用，并研究了其潜在机制。我们的结果显示，BMSC 衍生的外泌体具有外泌体的典型形态特征。LIPUS 介导的 BMSC 衍生的外泌体促进软骨再生，增加软骨细胞增殖和细胞外基质合成，抑制炎症，并抑制白细胞介素（IL）-1β诱导的核因子 kappa B（NF-κB）通路的激活。简而言之，LIPUS 增强了 BMSC 衍生的外泌体对骨关节炎软骨再生的促进作用，主要通过加强对炎症的抑制作用，并进一步增强软骨细胞增殖和软骨基质合成。其潜在机制可能与抑制 IL-1β诱导的 NF-κB 通路激活有关。
