School of Chemical Engineering, Sichuan University, Chengdu, P.R. China.
College of Physical Science and Technology, Sichuan University, Chengdu, P.R. China.
J Biomater Appl. 2021 Nov;36(5):772-788. doi: 10.1177/08853282211020399. Epub 2021 Jun 9.
In this study, tricalcium silicate (CS) calcium/polyphosphate/polyvinyl alcohol organic-inorganic self-setting composites were successfully designed. A variety of tests were conducted to characterize their self-setting properties, mechanical properties, degradation properties, and related biological properties. The composite bone cements showed a short setting time (5.5-37.5 min) with a 5:5-6:4 ratio of CS/CPP to maintain a stable compressive strength (28 MPa). In addition, PVA effectively reduced the brittleness of the inorganic phase. Degradation experiments confirmed the sustainable surface degradation of bone cement. A maximum degradation rate of 49% was reached within 56 days, and the structure remained intact without collapse. Culturing MC3T3 cells with bone cement extracts revealed that the composite bone cements had excellent biological properties . The original extract showed a proliferation promotion effect on cells, whereas most of the other original extracts of degradable bone cements were toxic to the cells. Meanwhile, extracellular matrix mineralization and alkaline phosphatase expression showed remarkable effects on cell differentiation. In addition, a good level of adhesion of cells to the surfaces of materials was observed. Taken together, these results indicate that CS/CPP/PVA composite bone cements have great potential in bone defect filling for fast curing.
在这项研究中，成功设计了硅酸三钙（CS）钙/多聚磷酸盐/聚乙烯醇有机-无机自凝复合材料。通过多种测试对其自凝特性、力学性能、降解性能和相关生物性能进行了表征。复合骨水泥的凝固时间较短（5.5-37.5 分钟），CS/CPP 比例为 5:5-6:4 可保持稳定的抗压强度（28 MPa）。此外，PVA 有效地降低了无机相的脆性。降解实验证实了骨水泥的可持续表面降解。在 56 天内达到了最大降解率 49%，且结构完整，没有崩溃。用骨水泥浸提液培养 MC3T3 细胞表明，复合骨水泥具有良好的生物性能。原始浸提液对细胞具有促进增殖的作用，而大多数其他可降解骨水泥的原始浸提液对细胞有毒。同时，细胞外基质矿化和碱性磷酸酶表达对细胞分化显示出显著的效果。此外，还观察到细胞对材料表面具有良好的黏附水平。总之，这些结果表明 CS/CPP/PVA 复合骨水泥在快速固化的骨缺损填充方面具有很大的潜力。
