苏丹人群中白细胞介素-17A基因rs2275913多态性与类风湿关节炎易感性的关联
Association of interleukin-17A rs2275913 polymorphism with rheumatoid arthritis susceptibility in Sudanese population.
作者信息
Osman Rgda Mohamed, Noma Mounkaila, Ahmed Abdallah Elssir, Abdelbagi Hanadi, Omer Rihab Ali, Ali Musab M, Mohammed Alameen Ayman Ali, Edris Ali Mahmoud, Muneer Mohamed S, Siddig Omayma, Hassan Rowa, Ahmed Eiman Siddig, Hassan Lamis Ahmed, Bakheet Osama El Hadi, Ahmed Ayman, Mohamed Nouh Saad, Siddig Emmanuel Edwar
机构信息
University of Medical Sciences and Technology, Khartoum, Sudan.
Department of Molecular Biology, Nile University, Khartoum, Sudan.
出版信息
SAGE Open Med. 2021 May 31;9:20503121211020207. doi: 10.1177/20503121211020207. eCollection 2021.
OBJECTIVES
Rheumatoid arthritis is a chronic inflammatory autoimmune disease. This study aimed to determine the association of interleukin-17A-197G/A polymorphism with rheumatoid arthritis in Sudanese patients.
METHODS
A case-control study was conducted between March and December 2018. Clinical and demographic data of the study participants were collected and analyzed. Polymerase chain reaction restriction fragment length polymorphism molecular technique was done to investigate interleukin-17A-197G/A polymorphisms. All statistical tests were considered statistically significant when p < 0.05.
RESULTS
The study population included 266 participants aged between 1 and 85 years, with an average of 40 years, classified into 85 (31.2%) cases (mean age 48.5 ± 11.3 years), and 181 (68.8%) controls (mean age 35.3 ± 15.9 years). The interleukin-17A homozygote AA genotype was more frequent among the control group compared to the case group; 95 (52.5%) and 7 (8.2%), respectively. The homozygote GG and the heterozygote AG genotypes were proportionally not different among the cases and control groups; 13 (54.2%) and 11 (45.8%), and 65 (46.4%) and 75 (53.6%), respectively. According to the distribution of interleukin-17A genotypes, a statistically significant difference was observed among cases with the interleukin-17A AA and AG genotypes, p values 0.001 and 0.004, respectively. For the association interleukin-17A genotypes and family history a negatively significant association was reported (95% confidence interval, -0.219, p value = 0.001). There was also a negatively significant association of interleukin-17A genotypes and anti-cyclic citrullinated peptide (95% confidence interval, -0.141, p value = 0.002).
CONCLUSION
This study is the first study in Sudan established the association between interleukin-17A-197G/A (rs2275913) polymorphisms and susceptibly to rheumatoid arthritis. These findings appeal for further research in Sudan to investigate the exact role of IL-17A in immunopathology and disease severity among Sudanese rheumatoid arthritis.
目的
类风湿性关节炎是一种慢性炎症性自身免疫性疾病。本研究旨在确定苏丹患者白细胞介素-17A - 197G/A多态性与类风湿性关节炎之间的关联。
方法
于2018年3月至12月进行了一项病例对照研究。收集并分析了研究参与者的临床和人口统计学数据。采用聚合酶链反应-限制性片段长度多态性分子技术来研究白细胞介素-17A - 197G/A多态性。当p < 0.05时，所有统计检验均被认为具有统计学意义。
结果
研究人群包括266名年龄在1至85岁之间的参与者，平均年龄为40岁，分为85例（31.2%）病例组（平均年龄48.5±11.3岁）和181例（68.8%）对照组（平均年龄35.3±15.9岁）。与病例组相比，对照组中白细胞介素-17A纯合子AA基因型更为常见；分别为95例（52.5%）和7例（8.2%）。纯合子GG和杂合子AG基因型在病例组和对照组中的比例无差异；分别为13例（54.2%）和11例（45.8%），以及65例（46.4%）和75例（53.6%）。根据白细胞介素-17A基因型的分布，在白细胞介素-17A AA和AG基因型的病例之间观察到统计学显著差异，p值分别为0.001和0.004。对于白细胞介素-17A基因型与家族史的关联，报告了负向显著关联（95%置信区间，-0.219，p值 = 0.001）。白细胞介素-17A基因型与抗环瓜氨酸肽之间也存在负向显著关联（95%置信区间，-0.141，p值 = 0.002）。
结论
本研究是苏丹首次建立白细胞介素-17A - 197G/A（rs2275913）多态性与类风湿性关节炎易感性之间关联的研究。这些发现呼吁在苏丹进行进一步研究，以调查IL-17A在苏丹类风湿性关节炎免疫病理学和疾病严重程度中的确切作用。
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