Ghule Mahesh R, Ramteke Purushottam K, Ramteke Sahadeo D, Kodre Prasad S, Langote Amruta, Gaikwad Akshay V, Holkar Somnath K, Jambhekar Hemangee
Research and Development Division, Vasumitra Life Energies Pvt Ltd, Pune, Maharashtra 411009 India.
Department of Botany, Raja Shripatrao Bhagawantrao Mahavidyalaya, District Satara, Aundh, Maharashtra 415510 India.
3 Biotech. 2021 Jun;11(6):290. doi: 10.1007/s13205-021-02843-3. Epub 2021 May 23.
The use of chitosan as an alternative for fungicides has received more attention worldwide. Hence, this study aimed to evaluate in vitro and in vivo antifungal activity of chitosan against causing root rot in fenugreek. Chitosan treatments ranged from 0.1 to 2gL were tested against on to potato dextrose agar and in potato dextrose broth. The results revealed that increase in concentrations of chitosan significantly reduced growth, dried biomass, sporulation and spore germination of . The hyphal swellings and distortion of mycelia were induced by chitosan. Fenugreek seeds treated with chitosan at 2 gL and 0.5 gL showed reduced infection and increased seed germination, respectively. In pot and field studies, fenugreek seeds treated with chitosan at 2.0 gL greatly reduced root rot disease severity and also enhanced yield parameters. The activity of defence enzymes, such as chitinase, β-1, 3-glucanase and total phenol were increased in chitosan treated in fenugreek plants. This increased activity offered protection to fenugreek plants against to a greater extent. The results showed that chitosan could be used as inducer of defense response and has the potential of controlling fenugreek root rot disease.
壳聚糖作为杀菌剂替代品的应用在全球范围内受到了更多关注。因此，本研究旨在评估壳聚糖对导致胡芦巴根腐病的病原菌的体外和体内抗真菌活性。在马铃薯葡萄糖琼脂和马铃薯葡萄糖肉汤上，测试了浓度范围为0.1至2g/L的壳聚糖处理对该病原菌的作用。结果表明，壳聚糖浓度的增加显著降低了该病原菌的生长、干生物量、产孢和孢子萌发。壳聚糖诱导了该病原菌菌丝的肿胀和菌丝体的畸变。用2g/L和0.5g/L壳聚糖处理的胡芦巴种子分别显示出病原菌感染减少和种子萌发增加。在盆栽和田间试验中，用2.0g/L壳聚糖处理的胡芦巴种子大大降低了根腐病的严重程度，还提高了产量参数。壳聚糖处理的胡芦巴植株中，几丁质酶、β-1,3-葡聚糖酶和总酚等防御酶的活性增加。这种活性的增加在很大程度上为胡芦巴植株提供了对该病原菌的保护。结果表明，壳聚糖可作为防御反应的诱导剂，具有控制胡芦巴根腐病的潜力。
