Department of Endodontics, Faculty of Dentistry, Kirikkale University, Kirikkale, Turkey.
Department of Biochemistry, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey.
Int Endod J. 2021 Oct;54(10):1915-1924. doi: 10.1111/iej.13589. Epub 2021 Jul 12.
To evaluate in a laboratory study, the effect of different canal irrigant solutions and activation techniques on transforming growth factor (TGF-β1), insulin growth factor-1 (IGF-1), bone morphogenetic protein-7 (BMP-7) and vascular endothelial growth factor-A (VEGF-A) release levels from the dentine of extracted premolar teeth.
Seventy premolar teeth with single root and canal were used. The lengths of the root segments were standardized to 12 mm, and the root canals were prepared up to size 100 with hand files. All surfaces of the teeth were covered with nail polish except the inner root canal surface. The root canals were irrigated with 1.5% NaOCl. Ten teeth were allocated to the control group. The remaining sixty teeth were divided into 2 main groups according to the chelating agent used (17% EDTA, 10% Citric acid; CA) and 3 subgroups (n = 10) according to irrigation activation technique (conventional syringe irrigation (CSI), passive ultrasonic irrigation (PUI) and Er:YAG laser activation). After the activation procedure, the root segments were placed into eppendorf tubes containing 1 mL of phosphate-buffered saline solution and kept at 37℃. TGF-β1, IGF-1, BMP-7 and VEGF-A release levels from dentine were measured using the enzyme-linked immunosorbent assay (ELISA) method at 24 h and at day 7. The volume of root canals was calculated using cone-beam computed tomography. The growth factor levels were calculated in ng/mL except VEGF-A (pg/ml). Normality analysis of the data was evaluated with the Kolmogorov-Smirnov test. Statistical analysis was performed using the Mann-Whitney-U and Wilcoxon tests.
Regardless of the activation type and sampling time, EDTA caused significantly more IGF release than did CA, whereas EDTA and CA were equally effective for the release of the other growth factors. For either EDTA or CA, the lowest and highest growth factor release levels were observed in the CSI and Er:YAG laser groups, respectively (p < .05). All of the growth factors were released significantly more at 24 h than on day 7 (p < .05).
Irrigation activation techniques with EDTA or CA increased the release levels of all growth factors from the dentine of canal walls in extracted premolar teeth.
在实验室研究中评估不同根管冲洗液和激活技术对从提取的前磨牙牙本质中转化生长因子 (TGF-β1)、胰岛素样生长因子-1 (IGF-1)、骨形态发生蛋白-7 (BMP-7) 和血管内皮生长因子-A (VEGF-A) 释放水平的影响。
使用 70 颗具有单根和根管的前磨牙。将根段长度标准化为 12mm，并用手锉将根管预备至 100 号。除根管内表面外，所有牙齿表面均涂有指甲油。用 1.5%次氯酸钠冲洗根管。10 颗牙分配到对照组。其余 60 颗牙根据螯合剂的使用情况分为 2 个主要组（17%EDTA、10%柠檬酸；CA），根据冲洗激活技术（常规注射器冲洗（CSI）、被动超声冲洗（PUI）和 Er:YAG 激光激活）分为 3 个亚组（n=10）。激活程序后，将根段放入含有 1mL 磷酸盐缓冲盐水的 eppendorf 管中，并保持在 37℃。在 24 小时和第 7 天，使用酶联免疫吸附测定 (ELISA) 法测量牙本质中 TGF-β1、IGF-1、BMP-7 和 VEGF-A 的释放水平。使用锥形束计算机断层扫描计算根管容积。除 VEGF-A（pg/ml）外，生长因子水平以 ng/mL 计算。数据的正态性分析采用 Kolmogorov-Smirnov 检验。使用 Mann-Whitney-U 和 Wilcoxon 检验进行统计分析。
无论激活类型和采样时间如何，EDTA 引起的 IGF 释放明显多于 CA，而 EDTA 和 CA 对其他生长因子的释放效果相同。对于 EDTA 或 CA，CSI 和 Er:YAG 激光组的生长因子释放水平最低和最高（p<0.05）。与第 7 天相比，所有生长因子在 24 小时时的释放水平均显著增加（p<0.05）。
EDTA 或 CA 的冲洗激活技术增加了从提取的前磨牙根管壁牙本质中所有生长因子的释放水平。
