Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
Shandong Binzhou Anim Sci and Vet Med Acad, Binzhou 256600, PR China.
Poult Sci. 2021 Jul;100(7):101149. doi: 10.1016/j.psj.2021.101149. Epub 2021 Mar 22.
Cage layer fatigue is a common metabolic disease associated with a calcium and phosphorus imbalance, but recently we found this disease can be led by high fat diet. In order to elucidate the pathogenesis induced by a high fat diet, we randomly divided 88 White Shell Roman layers into 2 groups. There were 44 layers in each group. The control group was fed by a standard layer rations, and the high fat group was fed by completed rations mixing with 3% soybean oil. This study successfully constructed an animal model of osteoporosis caused by high fat. Bone samples were collected for bone mineral density, bone biomechanical properties which are all decreased at 26, 30, 34, and 38 wk old. We found the pathway of tryptophan-ERK-CREB from the perspective of metabonomics which promote the bone absorption. By metabolomics, we screened the significantly activated tryptophan pathway in high fat feed and detected the elevated tryptophan metabolite serum 5-HT at 26, 30, 34 and 38 wk old in the high fat group. At 38 wk old, we detected significantly elevated protein and mRNA levels of ERK/CREB/C-fos in bone tissue in the high fat group. So we concluded that high-fat were associated with a decrease in bone density and bone biomechanical index by disrupting tryptophan-5-HT-ERK1/2-CREB metabolism signaling pathways.
笼养蛋鸡疲劳症是一种常见的代谢疾病，与钙磷失衡有关，但最近我们发现这种疾病可能是由高脂肪饮食引起的。为了阐明高脂肪饮食引起的发病机制，我们将 88 只白色罗曼蛋鸡随机分为两组，每组 44 只。对照组饲喂标准蛋鸡日粮，高脂肪组饲喂添加 3%大豆油的全价日粮。本研究成功构建了高脂肪诱导骨质疏松症的动物模型。在 26、30、34 和 38 周龄时，收集骨样本进行骨矿物质密度、骨生物力学特性检测，均发现下降。我们从代谢组学的角度发现了色氨酸-ERK-CREB 途径，该途径促进了骨吸收。通过代谢组学，我们筛选出了高脂肪饲料中显著激活的色氨酸途径，并在高脂肪组中检测到了在 26、30、34 和 38 周龄时升高的色氨酸代谢物血清 5-HT。在 38 周龄时，我们在高脂肪组的骨组织中检测到 ERK/CREB/C-fos 的蛋白和 mRNA 水平显著升高。因此，我们得出结论，高脂肪饮食通过破坏色氨酸-5-HT-ERK1/2-CREB 代谢信号通路，与骨密度和骨生物力学指标的降低有关。
